HIV variants carry natural polymorphisms related to drug resistance (R-markers) fixed during viral evolution in the absence of antiretroviral therapy (ART) that may impact on drug susceptibility and resistance pathways. We aimed to identify the HIV type 2 (HIV-2) variant-specific R-markers at Pol in all available sequences from ART-naive subjects deposited in Los Alamos database according to reported HIV-2 drug resistance-associated mutations (DRMs) and report the performance of two online HIV-2 resistance interpretation tools (HIV2EU Tool and Stanford HIVdb Program for HIV-2) to detect them. From a total of 587 sequences, we found 23 R-markers in low frequency, in groups A, B, and G. Four were present in >10% of the sequences with no direct impact on antiretroviral susceptibility. HIV2EU Tool detected one, whereas Stanford program all four. Stanford new tool, although still under development, seems effective in detecting HIV-2 DRMs and may prove a useful tool for HIV-2 resistance interpretation when fully developed.

中文翻译：

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简短交流：HIV 2 型变异中自然抗逆转录病毒耐药相关突变的更新和 HIV 2 型耐药解释工具的差异

HIV 变体携带与药物抗性（R 标记）相关的天然多态性，在病毒进化过程中固定，而抗逆转录病毒疗法 (ART) 可能会影响药物敏感性和耐药性途径。我们旨在根据报告的 HIV-2 耐药性相关突变 (DRM) 和存储在洛斯阿拉莫斯数据库中的未接受过 ART 受试者的所有可用序列，在 Pol 鉴定 HIV 2 型 (HIV-2) 变异特异性 R 标志物。报告两个在线 HIV-2 耐药性解释工具（HIV2EU Tool 和 Stanford HIVdb Program for HIV-2）检测它们的性能。从总共 587 个序列中，我们在 A、B 和 G 组中发现了 23 个低频 R 标记。4 个存在于 >10% 的序列中，对抗逆转录病毒敏感性没有直接影响。HIV2EU 工具检测到一个，而斯坦福程序检测到了所有四个。