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A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation
Journal of Natural Products ( IF 3.3 ) Pub Date : 2021-02-15 , DOI: 10.1021/acs.jnatprod.0c01125 Hao Cui 1 , Xinying Zhu 1 , Zhaoguang Wang 1 , Jianping Fang 2 , Tao Yuan 1
Journal of Natural Products ( IF 3.3 ) Pub Date : 2021-02-15 , DOI: 10.1021/acs.jnatprod.0c01125 Hao Cui 1 , Xinying Zhu 1 , Zhaoguang Wang 1 , Jianping Fang 2 , Tao Yuan 1
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A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8–11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice. The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate. Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption. The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
中文翻译：
从魔芋中提取的纯化葡甘露聚糖通过增强丁酸盐生成和组蛋白 H3 和 H4 乙酰化来改善结肠粘膜屏障功能
以天然魔芋葡甘聚糖 (KGM) 为原料，制备了结构明确的魔芋葡甘聚糖低聚糖 (KGMOS)，该低聚糖具有较高的分子量和较窄的分子量分布（分子量范围为 3000 至 4000 Da，聚合度 (dp) 8–11），并在 C57BL/6 小鼠中研究了 KGMOS 对结肠功能的有益作用和分子机制。该结果首次揭示了 KGMOS 调节肠道微生物群组成以促进结肠丁酸盐的产生。丁酸产量的增加进一步增加了组蛋白 H3 和 H4 的乙酰化，从而增强了主要结肠粘蛋白基因Muc2的转录和粘蛋白元件的分泌，这代表了 KGM 寡糖消耗的新分子机制。研究结果表明，具有特定分子大小的魔芋寡糖具有非常理想的功能特性，并且可能通过促进结肠粘膜的屏障功能来改善肠道健康。
更新日期：2021-02-26
中文翻译：
从魔芋中提取的纯化葡甘露聚糖通过增强丁酸盐生成和组蛋白 H3 和 H4 乙酰化来改善结肠粘膜屏障功能
以天然魔芋葡甘聚糖 (KGM) 为原料，制备了结构明确的魔芋葡甘聚糖低聚糖 (KGMOS)，该低聚糖具有较高的分子量和较窄的分子量分布（分子量范围为 3000 至 4000 Da，聚合度 (dp) 8–11），并在 C57BL/6 小鼠中研究了 KGMOS 对结肠功能的有益作用和分子机制。该结果首次揭示了 KGMOS 调节肠道微生物群组成以促进结肠丁酸盐的产生。丁酸产量的增加进一步增加了组蛋白 H3 和 H4 的乙酰化，从而增强了主要结肠粘蛋白基因Muc2的转录和粘蛋白元件的分泌，这代表了 KGM 寡糖消耗的新分子机制。研究结果表明，具有特定分子大小的魔芋寡糖具有非常理想的功能特性，并且可能通过促进结肠粘膜的屏障功能来改善肠道健康。
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