Integrated theranostic nanoplatforms with biomarker recognition and photothermal- and photodynamic (PTT/PDT) therapy is in high demand but remains challenging. Herein, a “sense-and-treat” nanoplatform based on semiconducting polymer nanoparticles (SPNs) for ratiometric bioimaging of phospholipase D (PLD) activity and PTT/PDT combined therapy was proposed. Semiconducting polymer nanoparticles (PSBTBT NPs) serve not only as photothermal agents but also as fluorescent quenchers of Rhodamine B (Rhod B) through a PLD-cleavable linker. Chlorin e6 (Ce6) was used as a photodynamic agent and fluorescence reference. The obtained nanoplatform (PSBTBT-Ce6@Rhod NPs) showed high PDT efficiency and photothermal performance upon single laser irradiation. The PTT/PDT combined therapy achieved more efficient tumor inhibition results as compared with single treatments. In addition, the overexpressed biomarker PLD in tumor tissue will cleave Rhod, leading to the fluorescence recovery of Rhod B and thus allowing the activatable fluorescence imaging of tumor and targeted phototherapy.

中文翻译：

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基于半导体聚合物纳米粒子的智能“感官治疗”纳米平台，用于精确的光热-光动力联合治疗

具有生物标志物识别以及光热和光动力（PTT / PDT）疗法的集成治疗治疗学纳米平台的需求量很大，但仍具有挑战性。在本文中，提出了一种基于半导体聚合物纳米粒子（SPNs）的“感官治疗”纳米平台，用于磷脂酶D（PLD）活性和PTT / PDT联合疗法的比例生物成像。半导体聚合物纳米颗粒（PSBTBT NPs）不仅可以用作光热剂，而且还可以通过PLD裂解的连接基充当罗丹明B（Rhod B）的荧光猝灭剂。氯霉素e6（Ce6）用作光动力剂和荧光参照物。所获得的纳米平台（PSBTBT-Ce6 @ Rhod NPs）在单激光照射下显示出较高的PDT效率和光热性能。与单一治疗相比，PTT / PDT联合治疗获得了更有效的肿瘤抑制效果。此外，肿瘤组织中过度表达的生物标志物PLD会裂解Rhod，导致Rhod B的荧光恢复，从而使肿瘤的荧光成像和靶向光疗成为可能。