Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.

中文翻译：

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池合成细胞库的基于成像的屏幕

将遗传扰动映射到表型变化是生物学研究的核心。显微镜技术的进步改变了这些研究，但它们在很大程度上仅限于一次检查几个菌株或细胞系。与此同时，在相对容易地创建转基因细胞合成文库方面发生了一场革命。在这里，我们描述了结合这些强大工具来对池生成的菌株库进行活细胞成像以改进生物学发现的方法。