Development of Real-Time Analysis System of Selenium Ion in Solutions by Using Microextraction and Fluorescence Microscopy,ACS Earth and Space Chemistry

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Development of Real-Time Analysis System of Selenium Ion in Solutions by Using Microextraction and Fluorescence Microscopy
ACS Earth and Space Chemistry ( IF 2.9 ) Pub Date : 2021-02-15 , DOI: 10.1021/acsearthspacechem.0c00327
Aileen Brandt ₁ , Takehiko Tsukahara ₁

Affiliation

The development of advanced analytical methods for trace amounts of selenium (Se) ions in wastewater become a matter of great importance in avoiding biological and environmental burdens due to Se pollution. In this study, a simple microanalytical methodology was developed, enabling the rapid and highly efficient extraction, real-time monitoring in situ, and extraction kinetic analysis of Se(IV) ions. The microscale extraction and analysis was verified by means of fluorescence intensity changes of fluorogenic complex 4,5-benzopiazselenol (BPS) consisting of the coordination of Se(IV) with 2,3-diaminonaphthalene (DAN). After the optimal BPS formation conditions, including pH, temperature, and Se(IV)/DAN concentration ratio, were examined, the microscale extraction of BPS was carried out under aqueous and organic two-phase parallel flow regimes in Y-shaped microchannels. The fluorescence intensity profiles of BPS in the organic phase were scanned in situ along the flow direction in the microchannel by a fluorescence microscope. The residence time dependence of the BPS concentrations could be precisely detected in real time. The results show that the developed microsystem has not only a detection limit of a submicromolar level for Se(IV), which is sufficiently lower than the permeable limit concentration of selenium in wastewater, but also a large apparent extraction rate constant of approximately 0.3 s^–1 and a short time to reach equilibrium of approximately 20 s, compared to those of bulk-scale extraction. This would indicate a valuable technique for elucidating microfluidic extraction kinetics and for applying real-time, environmentally friendly monitoring of toxic selenium ions.

中文翻译：

微萃取和荧光显微镜技术开发溶液中硒离子实时分析系统

开发先进的分析方法以处理废水中的痕量硒（Se）成为避免硒污染造成的生物和环境负担的重要问题。在这项研究中，开发了一种简单的微观分析方法，可实现快速高效的萃取，原位实时监测以及Se（IV）离子的萃取动力学分析。微量提取和分析通过荧光复合物4,5-苯并吡唑硒醇（BPS）的荧光强度变化进行了验证，该复合物由Se（IV）与2,3-二氨基萘（DAN）配位组成。在检查了最佳的BPS形成条件（包括pH，温度和Se（IV）/ DAN浓度比）后，BPS的微量萃取是在Y型微通道中，在水相和有机相两相平行流状态下进行的。通过荧光显微镜沿微通道中的流动方向原位扫描有机相中BPS的荧光强度分布。BPS浓度的停留时间依赖性可以实时精确检测。结果表明，开发的微系统不仅具有亚微摩尔水平的Se（IV）检出限，该检出限远低于废水中硒的可渗透极限浓度，而且具有约0.3 s的大表观萃取速率常数 BPS浓度的停留时间依赖性可以实时精确检测。结果表明，开发的微系统不仅具有亚微摩尔水平的Se（IV）检出限，该检出限远低于废水中硒的可渗透极限浓度，而且具有约0.3 s的大表观萃取速率常数可以实时精确地检测BPS浓度的停留时间依赖性。结果表明，开发的微系统不仅具有亚微摩尔水平的Se（IV）检出限，该检出限远低于废水中硒的可渗透极限浓度，而且具有约0.3 s的大表观萃取速率常数与批量提取相比，要达到^–1且在短时间内达到约20 s的平衡。这将为阐明微流体萃取动力学和应用实时，环境友好的有毒硒离子监测提供有价值的技术。

更新日期：2021-03-18

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