ABSTRACT

Background: Oral microbiota is believed to play important roles in systemic diseases, including cancer. Methods: We collected oral samples (tongue, buccal, supragingival, and saliva) and pancreatic tissue or intestinal samples from 52 subjects, and characterized 16S rRNA genes using high-throughput DNA sequencing. Results: Bray–Curtis plot showed clear separations between bacterial communities in the oral cavity and those in intestinal and pancreatic tissue samples. PERMANOVA tests indicated that bacterial communities from buccal samples were similar to supragingival and saliva samples, and pancreatic duct samples were similar to pancreatic tumor samples, but all other samples were significantly different from each other. A total of 73 unique Amplicon Sequence Variants (ASVs) were shared between oral and pancreatic or intestinal samples. Only four ASVs showed significant concordance, and two specific bacterial species (Gemella morbillorum and Fusobacterium nucleatum subsp. vincentii) showed consistent presence or absence patterns between oral and intestinal or pancreatic samples, after adjusting for within-subject correlation and disease status. Lastly, microbial co-abundance analyses showed distinct strain-level cluster patterns among microbiome members in buccal, saliva, duodenum, jejunum, and pancreatic tumor samples. Conclusions: Our findings indicate that oral, intestinal, and pancreatic bacterial microbiomes overlap but exhibit distinct co-abundance patterns in patients with pancreatic cancer and other gastrointestinal diseases.

 抽象的

背景：口腔微生物群被认为在包括癌症在内的全身性疾病中发挥着重要作用。方法：我们收集了 52 名受试者的口腔样本（舌头、颊、龈上和唾液）和胰腺组织或肠道样本，并使用高通量 DNA 测序来表征 16S rRNA 基因。结果：布雷-柯蒂斯图显示口腔中的细菌群落与肠道和胰腺组织样本中的细菌群落之间存在明显的分离。 PERMANOVA 测试表明，口腔样本的细菌群落与龈上样本和唾液样本相似，胰管样本与胰腺肿瘤样本相似，但所有其他样本均存在显着差异。口腔和胰腺或肠道样本共有 73 个独特的扩增子序列变体 (ASV)。在调整受试者内相关性和疾病状态后，只有四种 ASV 显示出显着的一致性，并且两种特定细菌物种（Gemella morbillorum 和 Fusobacter nucleatum subsp. vincentii）在口腔和肠道或胰腺样本之间显示出一致的存在或不存在模式。最后，微生物共丰度分析显示口腔、唾液、十二指肠、空肠和胰腺肿瘤样本中微生物组成员之间存在不同的菌株水平聚类模式。结论：我们的研究结果表明，胰腺癌和其他胃肠道疾病患者的口腔、肠道和胰腺细菌微生物群重叠，但表现出不同的共丰度模式。