Abstract

Phytochemical investigation on the roots of Aconitum pseudostapfianum resulted in the isolation of three new aconitine–type C₁₉–diterpenoid alkaloids, pseudostapines A–C (1–3). Their structures were determined by spectral methods such as 1D and 2D (¹H-¹H COSY, HMQC, NOESY and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated alkaloids were tested in vivo for their antinociceptive potential. As a result, pseudostapine C (3) showed 2-fold more potent antinociceptive effect (ID₅₀ = 60.3 μmol/kg) than the positive control drugs aspirin and acetaminophen.

中文翻译：

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从乌头分离的抗伤害性 C19-二萜生物碱

摘要

对乌头根的植物化学研究导致分离出三种新的乌头碱 C _{19 型}二萜生物碱，假乌头碱A–C ( 1–3 )。除了高分辨率质谱之外，它们的结构还通过光谱方法确定，例如一维和二维（¹ H - ¹ H COSY、HMQC、NOESY和HMBC）NMR光谱。在体内测试了分离的生物碱的镇痛潜力。结果，伪斯泰平 C ( 3 ) 显示出比阳性对照药物阿司匹林和对乙酰氨基酚强 2 倍的镇痛作用 (ID ₅₀ = 60.3 μmol/kg)。