Salinity is one of the major abiotic stresses that lead to loss of billions of dollars in crop production worldwide. The growth of rice plant is severely affected and subsequently the yield is generally low in salt affected areas. Salinity affects rice primarily at the early vegetative stage by interfering with biochemical and physiological processes governing its growth and development. This review aims at summarising various morphological, physiological, biochemical, and molecular-based methods that are currently used in screening salt-tolerant rice genotypes at different growth stages with particular emphasis on screening of breeding lines, and also the effectiveness of these methods. Field and laboratory-based methods are described including visual screening of salt-induced injuries as per the IRRI’s standard evaluation system, salt-induced accumulation of ions, changes in the levels of photosynthetic pigments, antioxidant, and image-based visual quantification of injuries, and molecular markers-based screening, which are lengthy and cumbersome. Among these methods currently available, this review highlights IC₅₀ (50% inhibition concentration) estimation of shoot growth inhibition as a rapid and accurate method that can differentiate genotypes with the IC₅₀ difference of only a few mm NaCl for the initial screening of a large number of rice germplasm and breeding lines.

盐分是主要的非生物胁迫之一，导致全球范围内数十亿美元的农作物减产。水稻植物的生长受到严重影响，因此，在盐害地区，单产通常较低。盐度主要通过干扰植物生长和发育的生化和生理过程来影响水稻的早期生长。这篇综述旨在概述目前用于筛选不同生育阶段的耐盐水稻基因型的各种形态，生理，生物化学和分子生物学方法，尤其着重于育种系的筛选，以及这些方法的有效性。描述了基于现场和实验室的方法，包括根据IRRI的标准评估系统目视检查盐引起的伤害，盐诱导的离子蓄积，光合色素水平的变化，抗氧化剂以及基于图像的损伤视觉定量以及基于分子标记的筛查，这些过程既冗长又麻烦。在当前可用的这些方法中，此评论着重介绍了IC₅₀（抑制浓度为50％）估计芽生长抑制是一种快速，准确的方法，可以区分基因型，NaCl的IC ₅₀差仅为几m m NaCl，用于大量水稻种质和育种系的初步筛选。