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Application of 50K chip-based genetic map to QTL mapping of stem-related traits in wheat
Crop & Pasture Science ( IF 1.8 ) Pub Date : 2021-02-12 , DOI: 10.1071/cp20326
Pengbo Song , Xin Wang , Xiaoxiao Wang , Feng Zhou , Xiaowan Xu , Bingjin Wu , Jianxin Yao , Dongyun Lv , Mengyu Yang , Xiaopeng Song , Daojie Sun

Stem strength is critical for lodging resistance in wheat. The present study utilised a high-density genetic map generated with a 50K single-nucleotide polymorphism (SNP) chip to map quantitative trait loci (QTLs) for stem-related traits. The analysis involved 198 recombinant inbred lines (RILs) derived from the cross between bread wheat (Triticum aestivum L.) varieties Xinong1376 and Xiaoyan81. The RILs were planted in randomised complete blocks with two replicates at three locations. The constructed genetic map contained 13571 SNP markers spanning 3605.53 cM across the 21 wheat chromosomes. The genetic and physical positions of SNP markers in 28 linkage groups were approximately the same. Twelve major QTLs related to stem strength were detected for eight traits and were distributed on chromosomes 2D (three QTLs), 3A, 4B, 4D (three QTLs), 5A, 5B, 5D and 6B. Each QTL explained 0.6-34.6% of the phenotypic variation. Taking full advantage of the available resources for fine-mapping of these stable QTLs will benefit molecular marker-assisted breeding and facilitate the dissection of molecular regulatory mechanisms underlying the mechanical properties of the wheat stem.



中文翻译:

基于50K芯片的遗传图谱在小麦茎相关性状QTL定位中的应用

茎强度对于小麦的抗倒伏性至关重要。本研究利用由50K单核苷酸多态性(SNP)芯片生成的高密度遗传图谱来绘制茎相关性状的数量性状基因座(QTL)。分析涉及来自面包小麦(Triticum aestivum)杂交的198个重组自交系(RIL)L.)品种Xinong1376和Xiaoyan81。将RIL种植在随机完整的块中,在三个位置重复两次。所构建的遗传图谱包含13571个SNP标记,跨21个小麦染色体,跨越3605.53 cM。28个连锁组中SNP标记的遗传和物理位置大致相同。检测到了十二个与茎强度有关的主要QTL,涉及八个性状,并分布在2D(三个QTL),3A,4B,4D(三个QTL),5A,5B,5D和6B染色体上。每个QTL解释了0.6-34.6%的表型变异。充分利用可利用的资源来精细映射这些稳定的QTL,将有益于分子标记辅助育种,并有助于剖析小麦茎力学特性背后的分子调控机制。

更新日期:2021-02-15
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