Tanshinone IIA (TanIIA) has multiple biological functions and already been clinically used to treat many cardiovascular diseases. TanIIA is a photoactive molecule and can be excited by light to generate ³TanIIA*. Generation of ³TanIIA* by TanIIA photosensitization indicates that TanIIA may serve as a photosensitizer to bring photodynamic damage to organisms. Therefore, human choroidal melanoma MUM-2B cell was chosen as a superficial tumor model and the photodynamic effect of TanIIA on tumor cells was evaluated in this study. The results showed that TanIIA photosensitization could generate singlet oxygen in noncellular system. MTT, clone formation and wound-healing assays showed that the survival and migration of MUM-2B cells could be efficiently inhibited by TanIIA photosensitization. And then, laser confocal microscope and flow cytometry were used to try to elucidate related mechanism. It was found that TanIIA could pass through cellular membrane and preferably accumulate in nucleus. TanIIA photosensitization could efficiently induce cell apoptosis and necrosis, increase intracellular ROS levels, decrease mitochondria membrane potential, and lead to cell cycle arrest in G2/M phase. Our findings indicate that TanIIA photosensitization can exert remarkable toxicity on choroidal melanoma cells.

中文翻译：

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丹参酮IIA光敏作用对脉络膜黑色素瘤细胞的有效抗癌作用

丹参酮IIA（TanIIA）具有多种生物学功能，已在临床上用于治疗多种心血管疾病。TanIIA 是一种光敏分子，可被光激发生成³ TanIIA*。³代通过TanIIA光敏化的TanIIA*表明TanIIA可以作为光敏剂对生物体带来光动力损伤。因此，选择人脉络膜黑色素瘤 MUM-2B 细胞作为浅表肿瘤模型，并在本研究中评估了 TanIIA 对肿瘤细胞的光动力效应。结果表明，TanIIA光敏可以在非细胞系统中产生单线态氧。MTT、克隆形成和伤口愈合试验表明，TanIIA 光敏作用可以有效抑制 MUM-2B 细胞的存活和迁移。然后，利用激光共聚焦显微镜和流式细胞仪试图阐明相关机制。发现TanIIA可以穿过细胞膜并优选在细胞核中积累。TanIIA光敏作用可有效诱导细胞凋亡和坏死，增加细胞内 ROS 水平，降低线粒体膜电位，导致细胞周期停滞在 G2/M 期。我们的研究结果表明，TanIIA 光敏作用可以对脉络膜黑色素瘤细胞产生显着的毒性。