This research aimed to assess effects of fish meal under different storage conditions on growth and antioxidant capacity for juvenile grouper within eight weeks. The healthy fish samples (initial body weight = 18.75 ± 0.05 g) were irregularly distributed to 0.5 m³ barrels with 28 fish per barrel, fed with each diet in the three replicate groups. Fish meals (FMs) were stored at 4°C (experiment 1) and room temperature (30 ± 2°C, experiment 2) for 45, 90 and 135 days, and the data of VBN, HA, AV and TBA were detected. Seven iso‐nitrogenous and iso‐lipidic diets were prepared and denoted as fresh fish meal FFM (control group), 4‐FM45, 4‐FM90, 4‐FM135, RT‐FM45, RT‐FM90 and RT‐FM135, respectively. Prolonged storage time, the volatile base nitrogen, acid value, histamine and thiobarbituric acid contents of FM increased significantly (p < .05). The growth condition and feed utilization of experimental fish were not significantly affected (p > .05). Prolonged storage time, in experiment 1, the catalase (CAT), the superoxide dismutase (SOD) activities and the malondialdehyde (MDA) contents initially increased dramatically and then decreased (p < .05). The reactivity of CAT mRNA in FFM group was significantly higher than that in other treatment groups (p < .05). In experiment 2, CAT activity decreased significantly (p < .05), whereas the MDA contents increased sharply (p < .05). SOD activity and CAT expression in the liver initially increased and then decreased (p < .05). The mRNA level and enzyme activity of trypsin decreased (p < .05) in experiment 1 and 2. In summary, storage of FM (within 135 days) cannot improve the growth and feed utilization of juvenile grouper but weaken the antioxidant capacity of the liver.

中文翻译：

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不同贮藏条件下鱼粉对石斑鱼幼鱼生长，血清生化指标和抗氧化能力的影响

这项研究旨在评估八周内不同储存条件下鱼粉对少年石斑鱼生长和抗氧化能力的影响。健康鱼类样品（初始体重= 18.75±0.05 g）不规则地分布到0.5 m ³每桶28条鱼，在三个重复组中分别喂食每种饲料。鱼粉（FMs）在4°C（实验1）和室温（30±2°C，实验2）下存储45、90和135天，并检测了VBN，HA，AV和TBA数据。准备了7种等氮和等脂饮食，分别表示为新鲜鱼粉FFM（对照组），4-FM45、4-FM90、4-FM135，RT-FM45，RT-FM90和RT-FM135。延长储存时间，FM的挥发性碱氮，酸值，组胺和硫代巴比妥酸含量显着增加（p  <.05）。实验鱼的生长条件和饲料利用率没有受到显着影响（p > .05）。延长的储存时间，在实验1中，过氧化氢酶（CAT），超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量最初急剧增加，然后下降（p  <.05）。FFM组中CAT mRNA的反应性显着高于其他治疗组（p  <.05）。在实验2中，CAT活性显着降低（p  <.05），而MDA含量急剧增加（p  <.05）。肝脏中的SOD活性和CAT表达首先升高，然后降低（p  <.05）。胰蛋白酶的mRNA水平和酶活性下降（p 实验1和2中的<0.05）。总而言之，储存FM（135天之内）不能改善少年石斑鱼的生长和饲料利用率，但会削弱肝脏的抗氧化能力。