The omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are essential fatty acids for marine fish. This study aimed to evaluate different dietary n-3 LC-PUFA on the growth, intestinal health and microbiome in carnivorous marine fish Trachinotus ovatus. Three diets, respectively, supplemented with fish oil, EPA-enriched oil and DHA-enriched oil were fed juvenile T. ovatus for 10 weeks. The results showed that the three groups showed no difference in the growth, histology and digestive enzyme activities of anterior intestine. Moreover, the intestinal ACP and ALP activities and tnfɑ mRNA level were increased in the EPA-enriched group, whereas the serum diamine oxidase activity and intestinal lysozyme activity and malondialdehyde content were decreased in both EPA- and DHA-enriched groups. Meanwhile, these two diets also down-regulated the mRNA expression of Mucin 2, Mucin 13, hes1-b, Krueppel-like factor 4 and Occludin, and up-regulated the zonula occludens-1 mRNA levels in intestine. Moreover, the two groups significantly decreased the intestinal Vibrio abundance and increased the probiotic proportions, especially in the EPA-enriched group. These results indicate dietary n-3 LC-PUFA would facilitate normal intestinal function and probiotics profiles of T. ovatus, and the EPA-enriched group appears to be more positive.

中文翻译：

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不同日粮 n-3 lc-pufa 对金鲳 (Trachinotus ovatus) 生长、肠道健康和微生物群特征的评价

omega-3 长链多不饱和脂肪酸 ( n -3 LC-PUFA)，如二十二碳六烯酸 (DHA) 和二十碳五烯酸 (EPA)，是海洋鱼类的必需脂肪酸。本研究旨在评估不同膳食n -3 LC-PUFA 对肉食性海洋鱼类Trachinotus ovatus的生长、肠道健康和微生物组的影响。分别添加鱼油、富含 EPA 的油和富含 DHA 的油的三种饮食被喂食卵形 T.幼鱼10 周。结果表明，三组在前肠的生长、组织学和消化酶活性方面均无差异。此外，肠道 ACP 和 ALP 活性和tnfɑ富含 EPA 的组的 mRNA 水平增加，而富含 EPA 和 DHA 的组的血清二胺氧化酶活性和肠道溶菌酶活性和丙二醛含量均降低。同时，这两个饮食也下调的mRNA表达粘蛋白2，粘蛋白13，HES1-B ，Krueppel样因子4和Occludin蛋白和上调的紧密连接-1在肠mRNA水平。此外，两组显着降低了肠道弧菌丰度并增加了益生菌比例，尤其是在富含 EPA 的组中。这些结果表明饮食n-3 LC-PUFA 将促进T. ovatus 的正常肠道功能和益生菌谱，富含 EPA 的组似乎更积极。