Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower specificity and sensitivity. In this review, we provide a summary of reaction additives and techniques that enhance the sensitivity, specificity, stability, robustness, or speed of isothermal nucleic acid amplification reactions used for sensing applications. We also provide the mechanism and purpose of each enhancement technique, which can provide guidance when selecting reaction additives to optimize novel isothermal amplification systems and assays.

等温核酸扩增技术用于检测各种分子，例如RNA，DNA和蛋白质。与金标准聚合酶链反应相比，等温方法快速简便，但通常具有较低的特异性和敏感性。在这篇综述中，我们提供了反应添加剂和技术的摘要，这些添加剂和技术可增强用于传感应用的等温核酸扩增反应的敏感性，特异性，稳定性，耐用性或速度。我们还提供了每种增强技术的机制和目的，可为选择反应添加剂以优化新型等温扩增系统和测定方法提供指导。