This paper reports the development of a novel probe based on magnetic room-temperature phosphorescence quantum dots with molecularly imprinted polymers (MQD-MIPs) for the rapid detection of trace norfloxacin (NFX) residual in complex food matrix. The highly selective probe was constructed by surface molecular imprinting technology using magnetic materials (Fe₃O₄ nanoparticles) as core, Mn-doped ZnS quantum dots (Mn-ZnS QDs) as phosphorescent materials, NFX as template, 3-aminopropyltriethoxysilane as functional monomer, and tetraethoxysilane as crosslinking agent. The as-obtained MQD-MIPs were characterized in detail by transmission electron microscopy, scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectrometry, and vibrating sample magnetometer. A magnetic strength of 37.64 emu g⁻¹ was recorded. Also, the probe displayed excellent room temperature phosphorescence properties with excitation/emission peaks at 300/590 nm. Under the optimized conditions, the detection time was less than 40 min, phosphorescence intensity varied linearly with concentration from 1 to 90 μg·L⁻¹, and detection limit reached as low as 0.80 μg·L⁻¹. Furthermore, the MQD-MIPs-based probe successfully detected norfloxacin residues in spiked fish and milk samples with recoveries of 90.92–111.53% and RSD <7%, outperforming the standard control method-HPLC-FLD (recoveries of 85.89–118.28%).

本文报道了一种基于磁性室温磷光量子点和分子印迹聚合物（MQD-MIPs）的新型探针的开发，该探针可用于快速检测复杂食品基质中的痕量诺氟沙星（NFX）残留。高选择性探针是通过表面分子印迹技术使用磁性材料（Fe ₃ O ₄以纳米粒子为核心，以Mn掺杂的ZnS量子点（Mn-ZnS QDs）为磷光材料，以NFX为模板，以3-氨基丙基三乙氧基硅烷为功能单体，以四乙氧基硅烷为交联剂。通过透射电子显微镜，扫描电子显微镜，X射线粉末衍射，傅立叶变换红外光谱法和振动样品磁力计对得到的MQD-MIP进行了详细表征。记录到37.64emug ^-1的磁强度。另外，该探针还具有出色的室温磷光性能，激发/发射峰位于300/590 nm。在最佳条件下，检测时间小于40 min，磷光强度随浓度从1到90μg·L ^-1线性变化。，检出限低至0.80μg·L ^-1。此外，基于MQD-MIPs的探针成功地检测了加标鱼肉和牛奶样品中的诺氟沙星残留，回收率90.92–111.53％，RSD <7％，优于标准对照方法HPLC-FLD（回收率85.89–118.28％）。