This study, investigates the interaction of bovine serum albumin (BSA) with synthesized chitosan nanoparticles (CSNPs) using steady-state fluorescence and UV–vis absorbance spectroscopy as well as picosecond time-resolved fluorescence technique. The fluorescence quenching mechanism of BSA by CSNPs indicates the presence of both static and dynamic mechanism. The loading efficiency of BSA-CSNPs exhibited a decrease by about 6% in neutral pH under physiological temperature. Transmission electron microcopy (TEM) images revealed the Synthesized CSNPs were irregular in shape with size of ~42 nm. The safety and biocompatibility of BSA-CSNPs inside the body was investigated after intraperitoneal (IP) injection of male mice for nine days, analysis of in vivo results, revealed no toxicity with a hypocholesterolemic effect and a predicted mild activation of WBCs due to CSNPs adjuvant and immunogenic peptides in BSA. Accordingly, no signs of hypersensitivity were observed due to the administration of such formulations. The results can be used for a better understanding the interaction of CSNPs within biological protein environment.

这项研究使用稳态荧光和紫外可见吸收光谱以及皮秒时间分辨荧光技术，研究了牛血清白蛋白（BSA）与合成的壳聚糖纳米颗粒（CSNPs）的相互作用。CSNP对BSA的荧光猝灭机制表明存在静态和动态机制。在生理温度下，BSA-CSNP的负载效率在中性pH值下降低了约6％。透射电子显微镜（TEM）图像显示，合成的CSNP形状不规则，尺寸约为42 nm。在腹膜内（IP）雄性小鼠腹腔注射9天后，研究了BSA-CSNP在体内的安全性和生物相容性，并对体内结果进行了分析，在BSA中没有发现具有降胆固醇作用和预期的WBC轻度激活的毒性，这归因于CSNP的佐剂和免疫原性肽。因此，由于施用此类制剂，未观察到超敏反应的迹象。该结果可用于更好地了解生物蛋白质环境中CSNP的相互作用。