Herein, fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) with red emission were synthesized and utilized as novel probe to detect D-penicillamine (D-Pen) for the first time. D-Pen molecules contain a thiol which can combine with Ag to form a non-fluorescent ground state complex, inducing the aggregation of DNA-AgNCs followed by the fluorescence quenching. The quenching mechanism is well-studied and found to be a static quenching process. This method can detect D-Pen in the range of 0.025–0.7 μM with the detection limit as low as 8 nM, which is 1–3 orders of magnitude more sensitive than those based on other fluorescent nanoprobes. More importantly, the preparation procedure for DNA-AgNCs is fast and without the requirement of heavy metal ions. Thus, this detection strategy is time-saving and eco-friendly. Satisfactory recoveries have been acquired for monitoring D-Pen in human serum samples and pharmaceutical samples owing to the high sensitivity.

本文中，合成了具有红色发光的荧光DNA模板银纳米簇（DNA-AgNCs），并首次用作检测D-青霉胺（D-Pen）的新型探针。D-Pen分子包含可以与Ag结合形成非荧光基态复合物的硫醇，诱导DNA-AgNCs聚集，然后进行荧光猝灭。对淬火机理进行了深入研究，发现它是静态淬火过程。这种方法可以检测D-Pen，范围为0.025-0.7μM，检测极限低至8 nM，比基于其他荧光纳米探针的D-Pen灵敏度高1-3个数量级。更重要的是，DNA-AgNCs的制备过程快速且不需要重金属离子。因此，这种检测策略既省时又环保。