The present study describes the biosynthesis of silver nanoparticles, using the fungus Penicillium verrucosum. The silver nanoparticles were synthesised by reacting silver nitrate (AgNO₃) with the cell free filtrates of the fungal culture, and were then characterized by UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy, energy-dispersive, and X-ray diffraction analysis to further evaluate their successful biosynthesis, optical and morphological features (size and shape), and crystallinity. The bioactivity of the synthesized nanoparticles against two phytopathogenic fungi i.e: Fusarium chlamydosporum and Aspergillus flavus was evaluated using nanomaterial seeding media. These biogenic silver nanoparticles were polydisperse in nature, with a size of 10–12 nm. With regard to the antifungal activity, 150 ppm of the nanoparticles suppressed the growth of F. chlamydosporum and A. flavus by about 50%. To the best of our knowledge, this is the first report on the use of P. verrucosum to synthesise silver nanoparticles. The present study demonstrates a novel, simple, and eco-friendly process for the generation of biofunctionally useful biogenic nanoparticles.

本研究描述了使用真菌青霉菌的银纳米粒子的生物合成。通过硝酸银（AgNO ₃）与真菌培养物的无细胞滤液反应合成银纳米颗粒，然后通过紫外可见光谱、透射电子显微镜、扫描电子显微镜、能量色散和X射线衍射进行表征分析以进一步评估其成功的生物合成、光学和形态特征（大小和形状）以及结晶度。使用纳米材料播种介质评估了合成的纳米颗粒针对两种植物病原真菌（即：厚垣镰刀菌和黄曲霉）的生物活性。这些生物银纳米颗粒本质上是多分散的，尺寸为 10-12 nm。在抗真菌活性方面，150 ppm 的纳米颗粒可抑制厚垣镰孢菌和黄曲霉的生长约 50%。据我们所知，这是第一份关于使用疣状疣合成银纳米粒子的报告。本研究展示了一种新颖、简单且环保的工艺，用于生成具有生物功能的生物纳米颗粒。