Clostridium butyricum TM-9A strain was employed as pure strain for scale up of fermentative hydrogen production in batch mode. In laboratory scale batch fermentations, 46 mmol/L hydrogen produced from molasses at optimum pH, molasses concentration and C/N ratio of; 7.5, 2.5%, and 10, respectively. Scale up of molasses fermentation by TM-9A strain in 13.5 L scale bioreactor under decreased partial pressure at regulated pH, enhnaced the hydrogen titer titer from 46 mmol H₂/L (in laboratory scale) to 73 mmol H₂/L (1.58 fold increase). Hydrogen productivity was 1650 mL/L. Scale up of glucose fermentation by TM-9A in 13.5 L scale bioreactor at optimumcondition produced; 71.9 mmol H₂/L (1634 mL/L). The biogas was composed of 65–60% H₂ and 35–40% CO_2. Hydrogen yield of TM-9A from glucose and molasses in the proto scale fermentation process was, 3.335 mole H₂/mole glucose and 73 mmol H₂/9 g of COD reduced, respectively. These studies imply that Clostridum butyricum TM-9A strain has significant potential for hydrogen production from spent organic matter in pilot scale.

丁酸梭菌TM-9A菌株被用作纯菌株，以分批方式扩大发酵氢的生产。在实验室规模的分批发酵中，在最佳pH，糖蜜浓度和C / N比的条件下，糖蜜会产生46 mmol / L的氢气；分别为7.5、2.5％和10。在调节的pH值下，通过降低分压在13.5 L规模生物反应器中通过TM-9A菌株发酵的糖蜜规模扩大，将氢滴定度从46 mmol H ₂ / L（实验室规模）提高到73 mmol H ₂ /L（1.58倍）增加）。氢气生产率为1650 mL / L。在最佳条件下，通过TM-9A在13.5 L规模的生物反应器中扩大葡萄糖发酵的规模；71.9 mmol H ₂ / L（1634 mL / L）。沼气由65–60％H _2组成和35-40％的CO _2，从葡萄糖和糖蜜在原规模的发酵过程TM-9A的氢气产率是，3.335摩尔ħ ₂ /摩尔葡萄糖和73毫摩尔ħ ₂ /9克COD的减少，分别。这些研究表明，丁酸梭菌TM-9A菌株具有在中试规模的废有机物中产氢的巨大潜力。