Clofibric acid, the main pharmacologically-active metabolite of pharmaceutical products used as antilipaemic agent has received, in last years, an increased interest because of its well-known recalcitrance to biodegradation and its high persistence in the aquatic environment. This molecule passes unchanged or poorly transformed in wastewater treatment plants. An indigenous strain of Streptomyces, named MIUG 4.89 was previously selected, exhibited the ability to favor clofibric acid biodegradation within submerged cultivation in controlled biotechnological conditions. Thus, in order to enhance the biodegradation of this refractory molecule, mathematical modeling and statistical optimization designs associated to Plackett-Burman Design and Response Surface Methodology were used to evaluate and optimize the effects of different major culture conditions of this bacterial isolate. According to the results, under optimized culture conditions (5 g L^-1 glucose, inoculation level 4.7%, 27.5 °C and 20 days of incubation) the strain Streptomyces MIUG 4.89 exhibited a successful removal of clofibric acid with a biodegradation yield of 54%, which is in agreement with model prediction. Thus, under optimized conditions, the removal yield was enhanced, which is very promising accounting for the refractory character of this water pollutant.

近年来，作为抗血脂药的药物产品的主要药理活性代谢产物氯纤维酸，由于其众所周知的抗生物降解性和在水生环境中的高度持久性而受到越来越多的关注。该分子在废水处理厂中通过不变或转化不良的分子。链霉菌的本地菌株先前选择的名为MIUG 4.89的产品在受控的生物技术条件下，具有在淹没栽培中促进氯纤维酸生物降解的能力。因此，为了增强该难降解分子的生物降解性，使用了与Plackett-Burman设计和响应面方法学相关的数学建模和统计优化设计来评估和优化该细菌分离株不同主要培养条件的影响。根据结果​​，在优化的培养条件下（5 g L ^-1葡萄糖，接种水平4.7％，27.5°C和孵育20天），菌株StreptomycesMIUG 4.89展示了成功去除氯纤维酸的生物降解率达54％，这与模型预测相符。因此，在优化的条件下，去除率得到了提高，这对于解决这种水污染物的难熔特性非常有希望。