The monogenean Benedenia humboldti is a pathogen of the yellowtail Seriola lalandi in the South-Eastern Pacific ocean. Using low-coverage short Illumina 150 bp pair-end reads sequencing, this study examines, for the first time, the ‘repeatome’ (= repetitive genomic elements), including the 45S ribosomal RNA DNA operon and microsatellites, in B. humboldti. Repetitive elements comprised a large fraction of the nuclear genome and a considerable proportion of them could not be assigned to known repeat element families. Taking into account only annotated repetitive elements, the most frequent belonged to the 45S ribosomal RNA operon or were classified as satellite DNA and Class I - Long Interspersed Nuclear Elements (LINEs) which were considerably more abundant than Class I - LTR elements. The ribosomal RNA gene operon in B. humboldti is comprised of, in the following order, a 5′ ETS (length = 233 bp), ssrDNA (2082 bp), ITS1 (346 bp), 5.8S rDNA (150 bp), ITS2 (572 bp), lsrDNA (3887 bp), and a 3′ ETS (1097 bp). A total of 15 SSRs were identified. These newly developed genomic resources will contribute to the better understanding of meta-population connectivity in this species, cryptic species in the genus, and will advance pest management strategies.

monogenean Benedenia humboldti是东南太平洋黄尾鰤Seriola lalandi的病原体。本研究使用低覆盖率的短 Illumina 150  bp 对端读取测序，首次检查了B. humboldti 中的“重复组”（= 重复基因组元素），包括 45S 核糖体 RNA DNA 操纵子和微卫星. 重复元件占核基因组的很大一部分，其中相当大的比例无法分配给已知的重复元件家族。仅考虑注释的重复元件，最常见的属于 45S 核糖体 RNA 操纵子或被归类为卫星 DNA 和 I 类 - 长散布核元件 (LINE)，它们比 I 类 - LTR 元件丰富得多。B. humboldti 中的核糖体 RNA 基因操纵子按以下顺序由 5' ETS（长度 = 233 bp）、ssrDNA（2082 bp）、ITS1（346 bp）、5.8S rDNA（150 bp）、ITS2（572 bp）、lsrDNA（3887 bp）组成) 和 3' ETS (1097 bp)。共确定了 15 个 SSR。这些新开发的基因组资源将有助于更好地了解该物种的元种群连接性，该属中的隐性物种，并将推进害虫管理策略。