The valorization of brewer’s spent grain (BSG), the main solid waste from this industry, is a topic of concern. The structural modifications generated by physicochemical pretreatments (autohydrolytic, H₂O₂ and H₂SO₄) to promote enzymatic accessibility and thus exploit this residue were assessed by Fourier transform infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy. After the pretreatments, a saccharification stage was conducted by an enzyme cocktail generated with high productivity (1.52 IU/(gds*d) filter paper activity, FPA, and 1.55 IU/(gds*d) endoglucanases, EGA, and 48.37 IU/(gds*d) xylanases) by Trichoderma reesei with BSG as substrate. Biomass recovery and sugar yields after pretreatments were consistent with hemicellulose and lignin removal and structural alterations generated. When the BSG was treated with H₂O₂ the enzymatic sugar release is efficient, obtaining 193.35 mg sugars/g dry BSG. Thus, conducting this pretreatment with H₂O₂ at low pH (5.0) and its combination with enzyme saccharification represents a promissory novel strategy to enhance the potential exploitation of this residue, using an economical and environmentally safe process.

啤酒糟（BSG）的增值是该行业的主要固体废物，这是一个令人关注的话题。通过傅里叶变换红外光谱，热重分析和扫描电子显微镜评估了通过物理化学预处理（自水解，H ₂ O ₂和H ₂ SO ₄）产生的结构修饰，以促进酶的可及性，从而利用了这种残留物。预处理后，通过高效率产生的酶混合物进行糖化阶段（1.52 IU /（gds * d）滤纸活性，FPA和1.55 IU /（gds * d）内切葡聚糖酶，EGA和48.37 IU /（ GDS * d）木聚糖酶）的里氏木霉以BSG为底物。预处理后的生物量回收和糖产量与半纤维素和木质素去除以及产生的结构变化一致。当用H ₂ O ₂处理BSG时，酶促糖释放是有效的，获得193.35 mg糖/ g干BSG。因此，在低pH（5.0）下用H ₂ O ₂进行预处理，并将其与酶糖化结合使用经济且对环境安全的方法，代表了一种新颖的策略，可以增强对该残留物的潜在利用。