Beauveria bassiana and Metarhizium anisopliae are two promising microbial agents for biopesticides development against the banana root borer Cosmopolites sordidus. In this study, germination, mycelial growth, and sporulation of six local Cameroonian isolates of those two species were assessed under seven different thermal conditions (13, 15, 20, 25, 29 33, and 37 °C) to select thermo-tolerant isolates. The Transmission potential of the thermo-tolerant isolates was determined at 25 ± 1 °C by dipping adult weevils in conidial suspensions (3.2 × 10⁸) conidia/ml and mixing these with uninoculated weevils in different proportions (0, 10, 30 and 50%), in groups of 30, and assessing the spread of the mycosis within the group over 35 d of co-incubation. Incubation temperature and isolates significantly affected germination, mycelial growth and conidial production. All isolates had large thermal tolerance ranges (13–33 °C) except MIITAC6.4.2 (20–29 °C). Horizontal transmission resulted in mortality of non-inoculated weevils from 4.63 ± 1.77 to 53.3 ± 11.9%. The isolate BIITAC6.2.2 exhibited high auto-dissemination potential and high conidia yield in cadavers. These results demonstrate the potential use of these isolates for biopesticides development against C. sordidus in Central Africa.

球孢白僵菌（Bevereria bassiana）和拟僵菌（Metarhizium anisopliae）是两种有前途的微生物制剂，可用于对抗香蕉根蛀虫Cosmopolites sordidus的生物农药。在这项研究中，在七个不同的热条件（13、15、20、25、29、33和37°C）下评估了这两个物种的六个喀麦隆本地分离株的发芽，菌丝生长和孢子形成，以选择耐热分离株。通过将成年象鼻虫浸入分生孢子悬浮液中，在25±1°C下确定耐热菌株的传播潜能（3.2×10 ⁸分生孢子/ ml，将它们与未接种的象鼻虫以不同的比例（0％，10％，30％和50％）混合，以30个为一组，并评估共同培养35 d内真菌病在该组中的传播。孵化温度和分离物显着影响发芽，菌丝生长和分生孢子的产生。除MIITAC6.4.2（20-29°C）外，所有菌株均具有较大的耐热范围（13-33°C）。水平传播导致未接种象鼻虫的死亡率从4.63±1.77降至53.3±11.9％。分离物BIITAC6.2.2在尸体中表现出高的自动传播潜力和高的分生孢子产量。这些结果证明了这些分离物在中部非洲对抗梭状芽胞杆菌的生物农药开发中的潜在用途。