Application of hyperosmolarity can be a promising strategy to promote chondrogenic differentiation in adipose-derived mesenchymal stem cells (ADSCs). Growth factors may promote different signaling pathways in parallel that is why in this study we monitor undesired pathologic or unwanted side effects as well as chondroinductive impacts of hyperosmolarity in differentiating ADSCs. Quantified gene expression, immunocytochemistry, glycosaminoglycan deposition and angiogenic secretion assays performed along with immunoassay. We observed that hyperosmolarity pressure of 480 mOsm over-expressed cartilage specific markers at gene expression level in the extra cellular matrix. Meanwhile, hyperosmolarity of 480 mOsm diminished the expression of cartilage associated pathologic markers, i.e., inflammatory and angiogenic attributes. Certain dose of hyperosmolarity could benefit chondrogenesis in a dual way, first by increasing chondrogenic markers and second by lowering tissue mineralization and angiogenic potential. The chondroprotective potential of hyperosmolarity could have a promising benefit in cartilage cell therapy and tissue engineering.

高渗透压的应用可能是促进脂肪来源间充质干细胞 (ADSCs) 软骨分化的一种有前景的策略。生长因子可能会同时促进不同的信号通路，这就是为什么在本研究中我们监测不希望的病理或不需要的副作用以及高渗在分化 ADSC 中的软骨诱导影响。与免疫测定一起进行的定量基因表达、免疫细胞化学、糖胺聚糖沉积和血管生成分泌测定。我们观察到 480 mOsm 的高渗压在细胞外基质中的基因表达水平上过度表达软骨特异性标记物。同时，480 mOsm 的高渗透压降低了软骨相关病理标志物的表达，即炎症和血管生成属性。一定剂量的高渗透压可以以双重方式有益于软骨形成，首先是通过增加软骨形成标志物，其次是通过降低组织矿化和血管生成潜力。高渗透压的软骨保护潜力可能在软骨细胞治疗和组织工程中具有广阔的前景。