Nanotechnology is a recent field of modern research dealing with synthesis, strategy and manipulation of particle’s structure in size range of 1–100 nm. This study introduces one of the methods of synthesis of nanoparticles, i.e., green synthesis of ZnO NPs using aqueous leaf extract of Becium grandiflorum (AM: ‘Yedegamentisie’). The biomolecules of the plant extract (such as phenols, flavonoids, saponins, glycosides, steroids, tannins and alkaloids) were used as capping and reducing agent during synthesis of ZnO NPs. Response surface methodology coupled with Box-Behnken design (RSM-BBD) was used to optimize the synthesis of ZnO NPs and adsorption studies of the as-synthesized ZnO NPs. Then, ZnO NPs was characterized using different spectroscopic and microscopic instruments such as UV–Vis spectroscopy, FTIR, XRD and SEM–EDS to consider its purity, shape and crystallinity. UV–Vis analysis showed peaks in the range 305–312 nm due to synthesis of ZnO NPs. FTIR analysis showed the availability of different phytochemicals in the plant extract and synthesis of ZnO NPs at 490 cm⁻¹. Powder XRD patterns confirmed formation of phase pure wurtzite structures of ZnO NPs. The synthesized ZnO NPs were used to remove MB dye from aqueous solution by acting as a photocatalyst and adsorbent as well as, it also showed antimicrobial activity against two gram positive (Staphylococcus epidermidis, Staphylococcus aureus) and three gram negative (Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa) bacteria.

纳米技术是现代研究的一个新领域，涉及尺寸范围为1–100 nm的粒子结构的合成，策略和操作。本研究介绍了一种纳米粒子的合成方法，即使用大叶铍的水性叶提取物绿色合成ZnO NPs。（AM：“ Yedegamentisie”）。植物提取物的生物分子（例如酚，类黄酮，皂角苷，糖苷，类固醇，单宁和生物碱）在合成ZnO NP时用作封端剂和还原剂。响应面方法结合Box-Behnken设计（RSM-BBD）用于优化ZnO NPs的合成和合成后ZnO NPs的吸附研究。然后，使用不同的光谱和显微镜仪器（例如UV-Vis光谱，FTIR，XRD和SEM-EDS）对ZnO NP进行表征，以考虑其纯度，形状和结晶度。UV-Vis分析显示，由于合成了ZnO NP，出现了305-312 nm范围内的峰。FTIR分析表明，在490 cm ^-1处植物提取物和ZnO NP的合成中可以使用不同的植物化学物质。粉末XRD图谱证实了ZnO NPs相纯纤锌矿结构的形成。合成的ZnO NPs用作光催化剂和吸附剂，用于去除水溶液中的MB染料，还显示出对2克阳性（表皮葡萄球菌，金黄色葡萄球菌）和3克阴性（大肠杆菌，克雷伯菌肺炎）的抗菌活性。，铜绿假单胞菌）细菌。