This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

中文翻译：

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在 Neurog1 +/- 缺陷小鼠中使用囊胚互补产生内耳感觉神经元

这项研究是第一个使用囊胚互补在 Neurog1+/- 杂合子小鼠中产生诱导多能干细胞衍生的内耳感觉神经元的研究。此外，这种方法纠正了与 Neurog1 杂合性相关的非感觉缺陷，表明互补是特定于内源性 Neurog1 功能的。这项工作验证了使用囊胚互补作为一种工具来创造对发育基因功能的新见解，并突出囊胚互补作为产生嵌合内耳细胞类型的潜在平台，这些细胞类型可以移植到受损的内耳中以改善听力。