ABSTRACT

Endophytic bacteria are those that colonise the internal tissues of plant without negative impact on plant health. This work aims to isolate the endophytic bacteria colonising Triticum vulgare and Phragmites australis and evaluate their compatibility as biostimulants to maize. Three of the six obtained isolates were selected upon their surpassing ability to stimulate the growth of maize seedlings (maximum of 18% increase in germination and 2.4-fold increase in the number of lateral roots). These isolates were molecularly identified as Klebsiella MK2R2, Bacillus B2L2 and Enterobacter E1S2. The response of maize seedlings to the combined effect of these isolates revealed that Bacillus B2L2, containing treatments, surpassed the other treatments in growth vigour parameters and upon this result these treatments were applied in a field experiment either alone or in combination with 100% N fertilisation. Maize plants, inoculated with Klebsiella MK2R2 and Bacillus B2L2 either alone or in combination with Enterobacter E1S2 in the presence of urea, surpassed significantly other treatments as the weight of ear increased almost two times and the number of grains increased 1.8 times compared to the control values. It could be concluded that the obtained strains are compatible biostimulants to maize.

摘要

内生细菌是定植在植物内部组织中而不会对植物健康造成负面影响的细菌。这项工作旨在分离定植在普通小麦和芦苇中的内生细菌，并评估它们作为玉米生物刺激剂的相容性。在获得的六个分离株中，有三个是根据其刺激玉米幼苗生长的能力来选择的（最大发芽量增加18％，侧根数增加2.4倍）。这些分离物在分子上鉴定为克雷伯菌MK2R2，芽孢杆菌B2L2和肠杆菌E1S2。玉米幼苗对这些分离物联合作用的反应表明芽孢杆菌含有B2L2的处理方法在生长活力参数方面超过了其他处理方法，根据这些结果，这些处理方法可单独或与100％N施肥一起用于田间试验。玉米植株单独接种或在尿素存在下接种克雷伯菌MK2R2和芽孢杆菌B2L2或与肠杆菌E1S2联合接种，大大超过了其他处理方法，因为耳重增加了近两倍，籽粒数量比对照增加了1.8倍。可以得出结论，所获得的菌株是与玉米相容的生物刺激剂。