ABSTRACT

A previous isolated Gordonia sp. (Lff) was used to degrade di-n-octyl phthalate (DOP) contamination in both aqueous solution and soil. The influence of temperature, pH, inoculum size, salt content and initial concentration of DOP on DOP degradation by Lff were analysed. The response of soil bacterial community to DOP and Lff was also analysed by Illumina MiSeq sequence method. Results showed that the optimal temperature, pH, inoculum size and salt content were 35^oC, 8.0, 5% and <5%, respectively. Under the optimal condition, more than 91.25% of DOP with different initial concentrations (100–2000 mg/L) could be degraded by Lff. Kinetics analysis indicated that biodegradation of DOP by Lff could be described by first-order kinetics (R² > 0.917) with the half-life (t_1/2) changing irregularly between 0.58 and 0.83 d. In addition, Lff enhanced the removal of DOP in soil and alleviated the toxicity of DOP on soil microorganisms. Furthermore, its influence on soil bacterial community is not obvious. These results suggested that Lff was effective in remediating DOP contamination in different environments.

摘要

以前孤立的Gordonia sp。(Lff) 用于降解水溶液和土壤中的邻苯二甲酸二正辛酯 (DOP) 污染。分析了温度、pH、接种量、盐含量和DOP初始浓度对Lff降解DOP的影响。还通过Illumina MiSeq测序方法分析了土壤细菌群落对DOP和Lff的响应。结果表明，最适温度、pH、接种量和盐含量分别为35 ^o C、8.0、5%和<5%。在最佳条件下，Lff可以降解91.25%以上不同初始浓度（100-2000 mg/L）的DOP。动力学分析表明 Lff 对 DOP 的生物降解可以用一级动力学 ( R ² > 0.917)，半衰期 ( t _1/2 ) 在 0.58 和 0.83 d 之间不规则变化。此外，Lff增强了土壤中DOP的去除，减轻了DOP对土壤微生物的毒性。此外，其对土壤细菌群落的影响并不明显。这些结果表明，Lff 可有效修复不同环境中的 DOP 污染。