This paper presents the development of a biosensor based on long period fiber grating, using a polyclonal antibody (OPN5) as a biological recognition element, which enables the detection of opsin (Opn5) in the brain tissue of a murine model with high specificity. Opsin (Opn5) is overexpressed in brain, ovarian, breast and prostate carcinomata and it may function as an important new biomarker to diagnose and monitor several types of cancer. In this study, the fiber surface was chemically prepared for antibody immobilization. The structural homogeneity of the biosensor was characterized by Fourier-transform infrared spectroscopy at each stage of the self-assembling process, and the response changes were measured by a signal transmission at the biosensor output. The biosensor developed was tested to detect Opn5 in brain tissue, and the interaction of Opn5 was observed through the evolution of the transmission spectrum in real time. Finally, the transmission data were analyzed by a principal component analysis (PCA).

本文介绍了一种基于长周期纤维光栅的生物传感器的开发，该传感器使用多克隆抗体（OPN5）作为生物识别元件，能够以高特异性检测鼠模型脑组织中的视蛋白（Opn5）。Opsin（Opn5）在脑，卵巢，乳腺癌和前列腺癌中过表达，它可能作为诊断和监测多种类型癌症的重要新生物标志物。在这项研究中，化学制备了纤维表面以固定抗体。在自组装过程的每个阶段，通过傅立叶变换红外光谱对生物传感器的结构均匀性进行表征，并通过生物传感器输出端的信号传输来测量响应变化。测试开发的生物传感器可检测脑组织中的Opn5，通过传输光谱的实时观察观察到Opn5的相互作用。最后，通过主成分分析（PCA）对传输数据进行分析。