Common hollyhock (Alcea rosea) is a ubiquitous ornamental in temperate climates but is highly adaptable and can be found growing in the tropics and subtropics. In 2019, an A. rosea plant showing symptoms of irregular chlorotic flecking on the basal leaves, with symptoms becoming gradually less severe toward the apex, was sampled in Pretoria, Gauteng province, South Africa. Total RNA was used to prepare an RNAtag-seq library, which was sequenced using an Illumina HiSeq 2500 instrument. Subsequent analysis of the data revealed the presence of two bipartite RNA viruses, namely orchid fleck virus (OFV) (segment 1: MW073772; segment 2: MW073773) and sweet potato chlorotic stunt virus (SPCSV) (segment 1: MW073774; segment 2: MW073775). OFV from this study was closely related to a strain from South Africa, associated with citrus leprosis disease, while SPCSV represented a novel strain. RT-PCR and bidirectional Sanger sequencing were used to confirm the presence of both viruses. Further samples were collected in 2020, which showed severe interveinal chlorosis, and were tested with RT-PCR; however only SPCSV was associated with these plants. This is the first time that both viruses have been associated with A. rosea, which should be considered a potential reservoir host of these agriculturally important viruses.

普通蜀葵（Alcea rosea）在温带气候中是一种普遍存在的观赏植物，但适应性强，可以在热带和亚热带地区发现。在2019年，玫瑰在南非豪登省比勒陀利亚取样了一种植物，该植物在基部叶片上显示出不规则的褪绿色斑点，症状逐渐减轻。总RNA用于制备RNAtag-seq文库，其使用Illumina HiSeq 2500仪器测序。随后的数据分析显示，存在两种两部分RNA病毒，即兰花斑点病毒（OFV）（第1部分：MW073772；第2部分：MW073773）和甘薯褪绿特技病毒（SPCSV）（第1部分：MW073774；第2部分： MW073775）。这项研究的OFV与南非的一种与柑橘麻风病有关的菌株密切相关，而SPCSV代表了一种新型菌株。RT-PCR和双向Sanger测序用于确认两种病毒的存在。2020年进一步收集了样本，表现出严重的静脉内萎黄，并通过RT-PCR进行检测；但是，只有SPCSV与这些植物相关联。这是两种病毒都首次关联玫瑰红曲霉，应被视为这些农业重要病毒的潜在宿主。