Currently, a vast majority of bacteria in the natural environment cannot be cultured or isolated under laboratory conditions mainly due to interdependence and interaction of cells. Quorum sensing (QS) is a cell-to-cell communication through which microbial cells regulate their biological activities. In this study, the interfacial sediments from Lake Taihu were induced by three types of signal compounds, including N-butanoyl-L-homoserine lactone (C4-HSL), autoinducer-2 (AI-2), and cyclic adenosine monophosphate (cAMP) under the laboratory conditions, and the variations in microbial diversity and composition after induction were investigated by culture-independent and culture-dependent methods, respectively. High-throughput sequencing analysis revealed that the dominant genera Vogesella and Cloacibacterium in the original sediment were supplanted by Aeromonas and Comamonas genera in response to AI-2 addition. However, the major microbial community in sediments induced by cAMP and C4-HSL was basically the same as that of the original sediment. The screen results indicated cAMP-like of Massilia strains and C4-HSL activity of Bacillus strains. Furthermore, cAMP and AI-2 stimulated Microbacterium strains, whereas C4-HSL and AI-2 promoted Arthrobacter. These findings together confirm the role of QS in mediating microbial community diversity and composition.

中文翻译：

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信号化合物对湖泊沉积物中微生物群落动态影响的文化依赖和文化独立分析

目前，自然环境中的绝大多数细菌无法在实验室条件下进行培养或分离，主要是由于细胞的相互依赖和相互作用。群体感应 (QS) 是一种细胞间通讯，微生物细胞通过该通讯调节其生物活动。本研究中，太湖界面沉积物受到三类信号化合物的诱导，包括N-丁酰基-L-高丝氨酸内酯（C4-HSL）、自诱导剂-2（AI-2）和环磷酸腺苷（cAMP）在实验室条件下，分别通过不依赖培养和依赖培养的方法研究了诱导后微生物多样性和组成的变化。高通量测序分析表明优势属原始沉积物中的Vogesella和Cloacibacterium被气单胞菌属和毛单胞菌属取代，以响应 AI-2 的添加。然而，cAMP和C4-HSL诱导的沉积物中主要微生物群落与原始沉积物基本相同。筛选结果表明，Massilia菌株具有cAMP 样活性，芽孢杆菌菌株具有C4-HSL 活性。此外，cAMP 和 AI-2 刺激了微杆菌菌株，而 C4-HSL 和 AI-2 促进了节杆菌。这些发现共同证实了 QS 在介导微生物群落多样性和组成方面的作用。