Diaphorina is a species-rich genus, native to the tropics and subtropics of the Old World, particularly of more arid regions. One of the species, Diaphorina citri, is the economically most important pest of citrus. Diaphorina species are morphologically similar which makes their identification difficult. In this study, the accuracy of DNA barcoding, using mitochondrial cytochrome c oxidase subunit 1 (COI), geometric morphometrics of the forewing and digital image processing methods were tested for identification of the three Diaphorina species: D. chobauti, D. citri and D. zygophylli. Moreover, the published COI sequences of D. citri, D. communis and D. lycii obtained from Genbank were used for cluster analyses. DNA barcodes for D. chobauti and D. zygophylli are deposited in Genbank for the first time. The results of the molecular and geometric morphometric analyses are congruent and place D. chobauti as the sister taxon of the other Diaphorina species. The geometric morphometric analysis shows that in D. zygophylli the fore margin is slightly curved proximally and sharply bent distally, while in D. chobauti and D. citri it is straight proximally and weakly bent distally. The results of digital image processing show that the distribution of the dark pattern differs consistently in the three studied species.

中文翻译：

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三种 Diaphorina 物种（半翅目：Liviidae）的分子、形态测量和数字自动识别

毛蕊花是一个物种丰富的属，原产于旧大陆的热带和亚热带，特别是更干旱的地区。物种之一，柑桔黄毛虫，是柑橘经济上最重要的害虫。毛蕊花物种在形态上相似，这使得它们的识别变得困难。在这项研究中，DNA 条形码的准确性，使用线粒体细胞色素 c 氧化酶亚基 1 (利益相关者)、前翅的几何形态计量学和数字图像处理方法进行了测试，以识别这三种毛蕊花物种：D. 乔巴蒂,D. 柑橘和D. zygophylli. 此外，已发表利益相关者序列D. citri, D.communis和枸杞从 Genbank 获得的用于聚类分析。DNA条形码用于D. 乔巴蒂和D. zygophylli首次存入Genbank。分子和几何形态学分析的结果是一致的D. 乔巴蒂作为另一个的姐妹分类单元毛蕊花物种。几何形态计量分析表明，在D. zygophylli前缘近端略微弯曲，远端急剧弯曲，而在D. chobauti 和 D. citri它近端笔直，远端微弯曲。数字图像处理结果表明，三个研究物种的暗纹分布始终存在差异。