Background: The use of phytochemicals for the treatment of various bodily ailments has been in practice since ancient days. Even though in practice, scientific studies on the protective effect of β-glucogallin (BG) against glaucoma is limited. Objectives: In the present study, the in vitro glaucoma model (hydrostatic pressure) using PC12 neuronal cells exposed to BG were used to elucidate its protective effects. Method: The cultured cells were analyzed for the mitochondrial responses, oxidant-antioxidant status, and expression of caveolin-1, ANGPTL7, the glaucoma markers, and cytokines. Results: We demonstrated a significant increase in the expression of glial fibrillary acidic protein, ANGPTL7, with altered mitochondrial enzymes in glaucoma cells compared to the control. Moreover, cells predisposed to hydrostatic pressure demonstrated an increase in oxidative stress with augmented (p #x3c; 0.01) inflammatory cytokines such as IL-2, CXCR4, IL-6, IL-8, MCP-1, and TNF-α. On the other hand, cells pretreated with BG attenuated the reactive oxygen species levels with improved antioxidant enzymes. Simultaneously, the levels of inflammatory cytokines and ANGPTL7 proteins were found attenuated with restored mitochondrial responses in BG pretreated cells. Conclusion: Thus, the results of the present study demonstrate that the use of BG on retinal cells against relieving the intraocular pressure may be a promising therapeutic for controlling the disease progression.
Neuroimmunomodulation

中文翻译：

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通过调节实验性青光眼的线粒体反应介导β-糖蛋白的抗青光眼潜力。

背景：自古以来，实践中就一直在使用植物化学物质来治疗各种身体疾病。即使在实践中，关于β-葡萄糖gallin（BG）对青光眼的保护作用的科学研究也很有限。目的：在本研究中，使用暴露于BG的PC12神经元细胞的体外青光眼模型（静水压）来阐明其保护作用。方法：分析培养的细胞的线粒体反应，氧化-抗氧化状态以及caveolin-1， ANGPTL7，青光眼标志物和细胞因子的表达。结果：我们证明，与对照相比，青光眼细胞中胶质纤维酸性蛋白ANGPTL7的表达随线粒体酶的改变而显着增加。此外，倾向于静水压力的细胞表现出氧化应激的增加，其炎症因子增加（p＃x3c; 0.01），例如IL-2，CXCR4，IL-6，IL-8，MCP-1和TNF-α。另一方面，用BG预处理的细胞通过改善的抗氧化剂降低了活性氧的水平。同时，发现在BG预处理的细胞中，随着恢复的线粒体反应，炎症细胞因子和ANGPTL7蛋白的水平减弱。结论：因此，本研究的结果表明，在视网膜细胞上使用BG对抗缓解眼压可能是控制疾病进展的有前途的疗法。
神经免疫调节