Scientific Data ( IF 5.8 ) Pub Date : 2021-02-11 , DOI: 10.1038/s41597-021-00842-w Cristian Gallardo-Escárate 1, 2 , Valentina Valenzuela-Muñoz 1, 2 , Gustavo Nuñez-Acuña 1, 2 , Diego Valenzuela-Miranda 1, 2 , Ana Teresa Gonçalves 1, 2 , Hugo Escobar-Sepulveda 1, 2 , Ivan Liachko 3 , Bradley Nelson 3 , Steven Roberts 4 , Wesley Warren 5
Caligus rogercresseyi, commonly known as sea louse, is an ectoparasite copepod that impacts the salmon aquaculture in Chile, causing losses of hundreds of million dollars per year. In this study, we report a chromosome-scale assembly of the sea louse (C. rogercresseyi) genome based on single-molecule real-time sequencing (SMRT) and proximity ligation (Hi-C) analysis. Coding RNAs and non-coding RNAs, and specifically long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were identified through whole transcriptome sequencing from different life stages. A total of 23,686 protein-coding genes and 12,558 non-coding RNAs were annotated. In addition, 6,308 lncRNAs and 5,774 miRNAs were found to be transcriptionally active from larvae to adult stages. Taken together, this genomic resource for C. rogercresseyi represents a valuable tool to develop sustainable control strategies in the salmon aquaculture industry.
中文翻译:
通过 SMRT 测序和 Hi-C 分析对海虱 Caligus rogercresseyi 进行染色体规模基因组组装
Caligus rogercresseyi,俗称海虱,是一种外寄生桡足类动物,影响智利的鲑鱼养殖业,每年造成数亿美元的损失。在这项研究中,我们报告了基于单分子实时测序(SMRT)和邻近连接(Hi-C)分析的海虱(C. rogercresseyi )基因组的染色体规模组装。通过不同生命阶段的全转录组测序鉴定了编码RNA和非编码RNA,特别是长非编码RNA(lncRNA)和微小RNA(miRNA)。总共注释了 23,686 个蛋白质编码基因和 12,558 个非编码 RNA。此外,还发现 6,308 个 lncRNA 和 5,774 个 miRNA 从幼虫到成虫阶段具有转录活性。总而言之,C. rogercresseyi的基因组资源是制定鲑鱼水产养殖业可持续控制策略的宝贵工具。