The aim of the study was to analyse the relationship between nutritional disorders and the expression of innate antibacterial response genes in patients admitted to the intensive care unit (ICU). In

46 patients with severe malnutrition and life-threatening surgical complications, nutritional status tests were performed on the basis of the NRS 2002 (Nutritional Risk Screening) scale, cytokine, albumin, C-reactive protein concentrations, anthropometric tests, and body composition analysis. Concurrently, the expression of Toll-like receptor 2, NOD1, TRAF6, and HMGB1 genes was determined in peripheral blood leukocytes at the mRNA level using real-time polymerase chain reaction. It was found that both the nutritional status and the gene expression changed depending on the group of patients studied (including the group of survivors vs. non-survivors). Significant correlations were found between the results of routine tests used in the diagnostics of malnutrition (including NRS 2002, resistance, reactance, phase angle, excess of extracellular water) and the expression of the studied genes. Moreover, the expression of TRAF6 and HMGB1 genes correlated with the Acute Physiology and Chronic Health Evaluation II scale and the age of the patients. The results of the research suggest that the expression of innate antibacterial response genes may be a new diagnostic tool complementing the assessment of nutritional disorders in surgical patients admitted to the ICU. These tests may be helpful in providing more accurate diagnostics of the genetic effects of malnutrition and in the monitoring of patients for whom nutritional treatment is planned to support the functions of the immune system, thereby increasing the effectiveness of this type of treatment in the ICU.

中文翻译：

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重症监护病房入院危重病患者的疾病相关营养不良与先天免疫基因表达之间的关联

该研究的目的是分析重症监护病房（ICU）患者的营养障碍与先天抗菌反应基因表达之间的关系。在

46名严重营养不良和危及生命的手术并发症的患者，在NRS 2002（营养风险筛查）量表，细胞因子，白蛋白，C反应蛋白浓度，人体测量学和身体成分分析的基础上进行了营养状况测试。同时，使用实时聚合酶链反应在外周血白细胞中以mRNA水平确定Toll样受体2，NOD1，TRAF6和HMGB1基因的表达。发现营养状况和基因表达均根据所研究的患者组（包括幸存者与非幸存者组）而改变。在营养不良诊断中使用的常规测试结果之间存在显着相关性（包括NRS 2002，电阻，电抗，相角，过量的细胞外水）和研究基因的表达。此外，TRAF6和HMGB1基因的表达与急性生理和慢性健康评估II量表和患者年龄相关。研究结果表明，先天抗菌反应基因的表达可能是一种新的诊断工具，可以补充对入住ICU的手术患者营养失调的评估。这些测试可能有助于提供营养不良的遗传影响的更准确诊断，并有助于监测计划进行营养治疗以支持免疫系统功能的患者，从而提高ICU中此类治疗的有效性。TRAF6和HMGB1基因的表达与急性生理和慢性健康评估II量表和患者年龄相关。研究结果表明，先天抗菌反应基因的表达可能是一种新的诊断工具，可以补充对入住ICU的手术患者营养失调的评估。这些测试可能有助于提供营养不良的遗传影响的更准确诊断，并有助于监测计划进行营养治疗以支持免疫系统功能的患者，从而提高ICU中此类治疗的有效性。TRAF6和HMGB1基因的表达与急性生理和慢性健康评估II量表和患者年龄相关。研究结果表明，先天抗菌反应基因的表达可能是一种新的诊断工具，可补充对入住ICU的手术患者营养失调的评估。这些测试可能有助于提供营养不良的遗传影响的更准确诊断，并有助于监测计划进行营养治疗以支持免疫系统功能的患者，从而提高ICU中此类治疗的有效性。研究结果表明，先天抗菌反应基因的表达可能是一种新的诊断工具，可以补充对入住ICU的手术患者营养失调的评估。这些测试可能有助于提供营养不良的遗传影响的更准确诊断，并有助于监测计划进行营养治疗以支持免疫系统功能的患者，从而提高ICU中此类治疗的有效性。研究结果表明，先天抗菌反应基因的表达可能是一种新的诊断工具，可以补充对入住ICU的手术患者营养失调的评估。这些测试可能有助于提供营养不良的遗传影响的更准确诊断，并有助于监测计划进行营养治疗以支持免疫系统功能的患者，从而提高ICU中此类治疗的有效性。