Introduction
To analyze the composition of subgingival biofilm and to assess the concentration of IL-1 and MMP-8 in gingival crevicular fluid (GCF) from deep periodontal pockets in patients with severe periodontitis to determine whether the presence of specific microbial species or the severity of the host’s immune response can be helpful in assessing the dynamics of disease.

Material and methods
The study included 30 individuals with periodontitis Grade B and 19 subjects with periodontitis Grade C. Quantitative and qualitative microbiological analysis of flora in pockets ≥ 7 mm was performed for the presence of selected periopathogens of the orange, red complex and Aggregatibacter actinomycetemcomitans using real-time PCR. The concentrations of IL-1 and MMP-8 in GCF were evaluated with the ELISA method.

Results
There were no differences in the composition of the subgingival biofilm depending on the diagnosis. The concentration of MMP-8 in GCF was significantly higher in periodontitis Grade C than in periodontitis Grade B (61 ng/µl and 37 ng/µl respectively, p = 0.039). The concentration of IL-1 was similar in both groups. No significant correlations were observed between the occurrence of individual periopathogens and concentrations of MMP-8 and IL-1 depending on the diagnosis.

Conclusions
Periodontitis grade may not be distinguished according to microbial analysis of subgingival biofilm or to concentration of IL-1in GCF. On the other hand, higher concentrations of MMP-9 in GCF from deep pockets may be helpful in detecting subjects particularly prone to occurrence and rapid progress of periodontitis.



中文翻译：

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牙周炎的进展速度与龈下生物膜成分或龈沟液中IL-1β和MMP-8浓度有关吗？

 介绍

分析龈下生物膜的组成并评估严重牙周炎患者深牙周袋龈沟液 (GCF) 中 IL-1 和 MMP-8 的浓度，以确定是否存在特定微生物种类或牙周病的严重程度宿主的免疫反应有助于评估疾病的动态。

 材料与方法

该研究包括 30 名患有 B 级牙周炎的个体和 19 名患有 C 级牙周炎的受试者。使用实时技术对≥ 7 mm 牙周袋中的菌群进行定量和定性微生物学分析，以确定橙色、红色复合体和放线菌聚合菌的选定周围病原体的存在PCR。采用ELISA法测定GCF中IL-1和MMP-8的浓度。

 结果

根据诊断的不同，龈下生物膜的成分没有差异。 C 级牙周炎 GCF 中 MMP-8 的浓度显着高于 B 级牙周炎（分别为 61 ng/μl 和 37 ng/μl，p = 0.039）。两组的 IL-1 浓度相似。根据诊断，个体周围病原体的发生与 MMP-8 和 IL-1 浓度之间没有观察到显着相关性。

 结论

根据龈下生物膜的微生物分析或 GCF 中 IL-1 的浓度可能无法区分牙周炎等级。另一方面，来自深牙袋的 GCF 中较高浓度的 MMP-9 可能有助于检测特别容易发生牙周炎并快速进展的受试者。