ABSTRACT

Recently, nanotherapy has been considered a promising and rapidly evolving field to combat cancer. Both gold nanoparticles and chitosan are interesting materials with biological and antitumor properties. The present study aimed to synthesise chitosan/gold nanocomposites (Cs/Au) using gamma irradiation technique and to study their anticancer, antibiofilm and antimicrobial activities. The synthesized Cs/Au nanocomposites were characterized using various analytical tools such as UV-vis, XRD, and TEM. UV-vis absorption spectra exhibited a surface plasmon resonance (SPR) peak at 525 nm that confirming the successful synthesis of colloidal AuNPs. The TEM image demonstrates well-dispersed spherical shaped Au nanoparticles. The cytotoxic activity of Cs/Au nanocomposites was examined against HepG-2 and CACO-2 cell lines in comparison to free chitosan using neutral red assay test. The Cs/Au nanocomposites inhibited the proliferation of cancer cells more than chitosan. Moreover, the prepared Cs/Au nanocomposites showed antibacterial and antibiofilm activities encouraging its potential candidature for biomedical and pharmaceutical purposes.

摘要

最近，纳米疗法被认为是对抗癌症的一个有前途且发展迅速的领域。金纳米粒子和壳聚糖都是具有生物和抗肿瘤特性的有趣材料。本研究旨在利用伽马辐照技术合成壳聚糖/金纳米复合材料（Cs/Au），并研究其抗癌、抗生物膜和抗菌活性。合成的 Cs/Au 纳米复合材料使用各种分析工具如 UV-vis、XRD 和 TEM 进行了表征。紫外-可见吸收光谱在 525 nm 处显示出表面等离子共振 (SPR) 峰，这证实了胶体金纳米粒子的成功合成。TEM 图像显示分散良好的球形金纳米颗粒。与游离壳聚糖相比，使用中性红测定法检测了 Cs/Au 纳米复合材料对 HepG-2 和 CACO-2 细胞系的细胞毒活性。Cs/Au纳米复合材料比壳聚糖更能抑制癌细胞的增殖。此外，制备的 Cs/Au 纳米复合材料显示出抗菌和抗生物膜活性，这鼓励了其潜在的生物医学和制药用途候选资格。