Abstract

Platelet-rich plasma (PRP) is a therapeutic option in different fields based on its growth factors. We investigated influence of PRP on islet survival, function, transplantation outcomes, and pancreatic genes expression in diabetic rats. In vitro: pancreatic isolated islets were incubated with/without PRP then viability, insulin secretion, and content were assessed. In vivo: Series 1 were designed to determine whether islet treatment with PRP improves transplantation outcome in diabetic rats by evaluating plasma glucose and insulin concentrations and oxidative parameters. Series 2, effects of PRP subcutaneous injection were evaluated on pancreatic genes expression and glucose tolerance test in diabetic rats. PRP enhanced viability and secretary function of islet. Reduced glucose and malondialdehyde levels as well as increased insulin levels, superoxide dismutase activity, and expressions of pdx1 and insulin were observed in diabetic rats. PRP treatment has positive effects on islet viability, function, transplantation outcome, and pancreatic genes expression in diabetic rats.

摘要

富血小板血浆 (PRP) 是基于其生长因子的不同领域的治疗选择。我们研究了 PRP 对糖尿病大鼠胰岛存活、功能、移植结果和胰腺基因表达的影响。体外：胰腺分离的胰岛在有/没有 PRP 的情况下孵育，然后评估活力、胰岛素分泌和含量。体内：系列 1 旨在通过评估血浆葡萄糖和胰岛素浓度以及氧化参数来确定用 PRP 进行胰岛治疗是否可以改善糖尿病大鼠的移植结果。系列 2，PRP 皮下注射对糖尿病大鼠胰腺基因表达和葡萄糖耐量试验的影响进行了评估。PRP增强了胰岛的活力和秘书功能。在糖尿病大鼠中观察到降低的葡萄糖和丙二醛水平以及增加的胰岛素水平、超氧化物歧化酶活性以及 pdx1 和胰岛素的表达。PRP 治疗对糖尿病大鼠的胰岛活力、功能、移植结果和胰腺基因表达有积极影响。