Abstract

This experiment was conducted to evaluate and compare the effects of a dietary inclusion with Aspergillus oryzae and Saccharomyces cerevisiae on productive performance, egg quality, intestinal morphology, and SGLT1 gene expression in laying Japanese quail. A total of 320 laying quails aged 47-day-old were randomly allocated to 8 treatments with 4 replicates of 10 quails each, for 7 weeks. The experimental diets consisted of a basal corn-soybean meal or control, control diet + 50 mg/kg tetracycline, control diet + 100 and 200 mg/kg A. oryzae, control diet + 100 and 200 mg/kg S. cerevisiae, control diet + 100 and 200 mg/kg of the combination of both A. oryzae and S. cerevisiae at equal proportions. At the end of the experiment, 4 quails per treatment were slaughtered, and tissue samples from the jejunum (1.5 cm in length) were removed for intestinal morphology measurements. Samples from the midsegment of the jejunum (150–200 mg) were taken to evaluate the gene expression of sodium-glucose cotransporter (SGLT1). The dietary inclusion of S. cerevisiae at 200 mg/kg significantly improved the feed conversion ratio (FCR) and egg production and reduced the feed intake (FI) (p < .001). The greatest shell thickness values were found in those fed on diets containing S. cerevisiae at 200 mg/kg. The quails fed on diets containing the combination of both probiotics at 100 mg/kg showed significantly higher egg weight (p < .001). Shell percent and yolk index increased significantly in quails fed on S. cerevisiae-supplemented diets at 100 mg/kg (p < .05). The villus height in the S. cerevisiae-supplemented group (200 mg/kg) was significantly higher than other groups (p < .05). The villus width was significantly higher in quails given 200 mg/kg A. oryzae compared to those received 100 mg/kg A. oryzae or 200 mg/kg S. cerevisiae (p < .05). Supplementation of S. cerevisiae at both tested levels resulted in significantly higher SGLT1 gene expression in the jejunum (p < .05). In conclusion, the results of this experiment demonstrated that among the two probiotics, S. cerevisiae at the level of 200 mg/kg as a functional feed additive has a better potential to improve productive performance, intestinal morphology, and SGLT1 gene expression of laying Japanese quails.

• HIGHLIGHTS

• Supplementation of diet with 200 mg/kg S. cerevisiae improved egg production, shell thickness, and FCR.

• The addition of 200 mg/kg S. cerevisiae to the diets improved the gut development by enhancing the villus height.

• Inclusion of diet with S. cerevisiae at both levels upregulated the gene expression of SGLT1.

摘要

进行该实验以评估和比较日粮中添加米曲霉和酿酒酵母对产蛋鹌鹑的生产性能，蛋品质，肠道形态和SGLT1基因表达的影响。总共将320只年龄为47天的鹌鹑鹌鹑随机分配到8个处理中，每组10个鹌鹑重复4次，共7周。实验饮食包括基础玉米-大豆粉或对照，对照饮食+ 50 mg / kg四环素，对照饮食+ 100和200 mg / kg米曲霉，对照饮食+ 100和200 mg / kg酿酒酵母，对照饮食+米曲霉和酿酒酵母的组合分别为100和200 mg / kg比例相等。实验结束时，每处理一次将4只鹌鹑宰杀，并从空肠（长1.5厘米）中取出组织样本进行肠道形态测量。空肠中段（150-200 mg）的样本用于评估钠-葡萄糖共转运蛋白（SGLT1）的基因表达。饮食中200 mg / kg酿酒酵母的加入显着提高了饲料转化率（FCR）和产蛋量，并降低了饲料采食量（FI）（p  <.001）。在饲喂含200 mg / kg啤酒酵母的日粮中发现最大的壳厚度值。两种益生菌含量为100 mg / kg的日粮混合鹌鹑表现出明显更高的蛋重（p <.001）。以100 mg / kg补充酿酒酵母（S. cerevisiae）补充日粮喂养的鹌鹑的壳百分比和蛋黄指数显着提高（p  <.05）。酿酒酵母补充组的绒毛高度（200 mg / kg）显着高于其他组（p  <.05）。给予200 mg / kg米曲霉的鹌鹑的绒毛宽度比接受100 mg / kg米曲霉或200 mg / kg酿酒酵母的鹌鹑的绒毛宽度显着更高（p  <.05）。在这两个测试水平上补充酿酒酵母会导致空肠中SGLT1基因表达显着升高（p <.05）。总之，该实验的结果表明，在两种益生菌中，以200 mg / kg的酿酒酵母作为功​​能性饲料添加剂具有更好的潜力，可改善产蛋鸡的生产性能，肠道形态和SGLT1基因表达鹌鹑。

• 强调

• 在日粮中添加200 mg / kg酿酒酵母可以提高产蛋量，蛋壳厚度和FCR。

• 日粮中添加200 mg / kg酿酒酵母通过增加绒毛高度来改善肠道发育。

• 在两个水平上将饮食与酿酒酵母一起包括上调了SGLT1的基因表达。