Accomplishing slow translocation speed with high sensitivity has been the most critical mission for solid‐state nanopore (SSN) device to electrically detect nucleobases in ssDNA. In this study, a method to detect nucleobases of ssDNA using a 2D SSN is introduced by considerably reducing the translocation speed and effectively increasing its sensitivity. The ultra‐thin titanium dioxide coated hexagonal boron nitride nanopore was fabricated, along with an ionic‐liquid 1‐butyl‐3‐methylimidazolium hexafluorophosphate/2.0 M KCl aqueous (cis/trans) interface, for increasing both the spatial and the temporal resolutions. As the ssDNA molecules entered the nanopore, a brief surge of electrical conductivity occurred, which was followed by multiple resistive pulses from nucleobases during the translocation of ssDNA and another brief current surge flagging the exit of the molecule. The continuous detection of nucleobases using a 2D SSN device is a novel achievement: the water molecules bound to ssDNA increased the molecular conductivity and amplified electrical signals during the translocation. Along with the experiment, computational simulations using COMSOL Multiphysics are presented to explain the pivotal role of water molecules bound to ssDNA to detect nucleobases using a 2D SSN.

中文翻译：

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通过具有离子-液体/盐-水界面的二维 h-BN 纳米孔检测水合 ssDNA 中的核苷酸

以高灵敏度实现缓慢的易位速度一直是固态纳米孔 (SSN) 装置电检测 ssDNA 中核碱基的最关键任务。在这项研究中，通过显着降低易位速度并有效提高其灵敏度，引入了一种使用 2D SSN 检测 ssDNA 核碱基的方法。制备了超薄二氧化钛涂层六方氮化硼纳米孔，以及离子液体 1-丁基-3-甲基咪唑鎓六氟磷酸盐/2.0 M KCl 水溶液（顺式/反式）) 接口，用于增加空间和时间分辨率。当 ssDNA 分子进入纳米孔时，会发生短暂的电导率激增，随后在 ssDNA 易位期间来自核碱基的多个电阻脉冲和另一个标志着分子退出的短暂电流浪涌。使用 2D SSN 设备连续检测核碱基是一项新成就：与 ssDNA 结合的水分子在易位过程中增加了分子电导率并放大了电信号。除了实验之外，还展示了使用 COMSOL Multiphysics 进行的计算模拟，以解释与 ssDNA 结合的水分子在使用 2D SSN 检测核碱基方面的关键作用。