The further development and application of capillary electromigration techniques for the enantioselective determination of drugs and their metabolites in body fluids, tissues, and in vitro preparations during the 2010 to 2020 time period continued to proof their usefulness and attractiveness in bioanalysis. This review discusses the principles and important aspects of capillary electrophoresis- based chiral drug bioassays, provides a survey of the assays reported during the past 10 years and presents an overview of the key achievements encountered in that time period. For systems with charged chiral selectors, special attention is paid on assays that feature field-amplified sample injection to enable the determination of ppb levels of analytes and optimized online incubation procedures for the rapid assessment of a metabolic pathway. Applications discussed encompass the pharmacokinetics of drug enantiomers in vivo and in vitro, the impact of inhibitors on metabolic steps, the elucidation of the stereoselectivity of drug metabolism in vivo and in vitro, and drug enantiomers in toxicological, forensic, and doping analysis.

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通过手性电迁移技术对药物及其代谢物进行生物分析（2010-2020）

毛细管电迁移技术在体液、组织和体外对映选择性测定药物及其代谢物的进一步发展和应用2010 年至 2020 年期间的准备工作继续证明它们在生物分析中的有用性和吸引力。本综述讨论了基于毛细管电泳的手性药物生物测定的原理和重要方面，对过去 10 年报告的测定进行了调查，并概述了该时期取得的主要成就。对于带有带电手性选择器的系统，需要特别注意具有场放大样品注射功能的分析，以便确定分析物的 ppb 水平，并优化在线孵育程序以快速评估代谢途径。讨论的应用包括药物对映体在体内和体外的药代动力学，抑制剂对代谢步骤的影响，阐明体内和体外药物代谢的立体选择性，以及毒理学、法医学和兴奋剂分析中的药物对映异构体。