In this study scaffolds of nanohydroxyapatite (nHA) and anionic collagen (C) combined with plant extracts intended for bone tissue repair were developed. Grape seed (P), pomegranate peel (R) and jabuticaba peel (J) extracts were used as collagen crosslinker agents in order to improve the materials properties. All crude extracts were effective against Staphylococcus aureus, but only for CR scaffold inhibition zone was noticed. The extracts acted as crosslinking agents, increasing enzymatic resistance and thermal stability of collagen. The extracts showed cytotoxicity at the concentrations tested, while nHA increased cell viability. The scaffolds presented porosity and pore size appropriate for bone growth. CR, CnHAP, CnHAR and CnHAJ increased the cell viability after 24 h. The combination of collagen, nHA and plant extracts offers a promising strategy to design novel biomaterials for bone tissue regeneration.

在这项研究中，开发了纳米羟基磷灰石（nHA）和阴离子胶原（C）的支架，以及旨在用于骨组织修复的植物提取物。葡萄籽（P），石榴皮（R）和茉莉果皮（J）提取物被用作胶原蛋白交联剂，以改善材料性能。所有粗提物均对金黄色葡萄球菌有效，但仅针对CR支架抑制区。提取物起到交联剂的作用，增加了胶原蛋白的抗酶活性和热稳定性。提取物在所测试的浓度下显示出细胞毒性，而nHA增加了细胞活力。支架具有适合骨骼生长的孔隙率和孔径。CR，CnHAP，CnHAR和CnHAJ在24小时后增加了细胞活力。胶原蛋白，nHA和植物提取物的组合为设计用于骨组织再生的新型生物材料提供了一种有前途的策略。