The ever-increasing ozone (O₃) concentration has led to reduced production and altered quality of soybean. Abundant reports have explored the damage mechanisms of O₃ on soybean. However, how the elevated O₃ affects metabolite profiling of soybean remains to be poorly understood. Here, we compare the metabolic profile of soybean leaves under charcoal filtered air (CF, <20 ppb) and short-term elevated O₃ concentration (EO, 100 ppb). High level of O₃ affects metabolites for the tricarbonic acid (TCA) cycle, reactive oxygen species, cell wall composition and amino acids. Significantly, jasmonic acid-related metabolite promoting stomata closure is highly induced with 125-fold change. Furthermore, O₃ fumigation alters the expression of genes contributing to the biosynthesis of certain metabolites in TCA cycle. Together, these findings identify a wide range of changed metabolites in response to O₃ pollution. Our results pave the way for the genetic improvement of soybean to adapt to O₃ pollution to maintain stable yields.

不断增加的臭氧（O ₃ ）浓度导致大豆产量减少和品质改变。大量报道探讨了O ₃对大豆的损害机制。然而，升高的 O ₃如何影响大豆的代谢谱仍知之甚少。在这里，我们比较了经木炭过滤的空气（CF，<20 id=138>3 浓度（EO，100 ppb））下大豆叶片的代谢特征。高水平的 O ₃影响三碳酸 (TCA) 循环的代谢物、活性氧值得注意的是，茉莉酸相关代谢物促进气孔闭合，并发生 125 倍的变化。此外，O ₃熏蒸还改变了 TCA 循环中某些代谢物生物合成的基因表达。 ，这些发现确定了针对 O ₃污染的广泛变化的代谢物，我们的结果为大豆的遗传改良以适应 O ₃污染以保持稳定的产量铺平了道路。