Introduction. Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. This study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients. Materials and Methods. In a case-control study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during 2018 and 2019, 23 and 11 gastric antral biopsy samples with H. pylori infection were collected from gastritis and gastric carcinoma patients by the consecutive and available sampling method. Pathological characters, including tumor grades and tumor areas for gastric carcinoma biopsy samples prepared from gastric cancer areas, were determined by the pathologist. Total RNA of gastric antral biopsy samples was extracted, and their cDNA was synthesized by TaKaRa kit. H. pylori virulence genes’ cDNA using specific primers and PCR was detected. This study’s results were analyzed by SPSS version 25 and statics chi-square tests for determination of relationship and correlation between cDNAs of H. pylori transcriptional profile and clinical outcomes of H. pylori infection, including gastritis, gastric carcinoma, tumor grades, and tumor area. Results. The positive statistical correlations were observed between transcripts of cagA, cagA-EPIYAC, cagE, and cagY genes and H. pylori infection clinical outcomes (). Conclusion. Detection of the H. pylori virulence genes’ cDNA in gastric biopsy samples can help provide the prognosis of clinical outcomes.

中文翻译：

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胃炎和胃癌患者幽门螺杆菌毒力基因的转录谱

简介。迄今为止，已经对幽门螺杆菌感染患者中幽门螺杆菌毒力基因的频率进行了大量分子流行病学研究。本研究旨在通过 cDNA 检测胃炎和胃癌患者胃活检样本中幽门螺杆菌毒力基因的转录谱。材料和方法。在一项病例对照研究中，根据 2018 年和 2019 年 Sanandaj 市胃炎和胃癌的患病率，23 份和 11 份胃窦活检样本含有幽门螺杆菌通过连续和可用的采样方法从胃炎和胃癌患者中收集感染。从胃癌区域制备的胃癌活检样本的病理特征，包括肿瘤等级和肿瘤区域，由病理学家确定。提取胃窦活检标本的总RNA，用TaKaRa试剂盒合成其cDNA。使用特异性引物和PCR检测H. pylori毒力基因的cDNA。本研究的结果通过 SPSS 第 25 版和静态卡方检验进行分析，以确定H. pylori转录谱的 cDNA 与 H. pylori临床结果之间的关系和相关性感染，包括胃炎、胃癌、肿瘤分级和肿瘤面积。结果。cagA、cagA-EPIYAC、cagE和cagY基因的转录本与H. pylori感染的临床结果呈正相关。）。 结论。在胃活检样本中检测幽门螺杆菌毒力基因的 cDNA 可以帮助提供临床结果的预后。