The inherent constraints on resolution, speed and field of view have hindered the development of high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses a series of axially distributed reflecting slits to probe different depths within a sample volume. Our technique enables the simultaneous imaging of an optically sectioned image stack with a single camera at frame rates of hundreds of hertz, without the need for axial scanning. We demonstrate the applicability of our system to monitor fast dynamics in biological samples by performing calcium imaging of neuronal activity in mouse brains and voltage imaging of cardiomyocytes in cardiac samples.

中文翻译：

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使用轴向分布狭缝的快速多平面线扫描共焦显微镜


分辨率、速度和视场的固有限制阻碍了大规模高速三维显微镜技术的发展。在这里，我们提出了一种多平面线扫描成像策略，该策略使用一系列轴向分布的反射狭缝来探测样本体积内的不同深度。我们的技术能够使用单个相机以数百赫兹的帧速率同时成像光学切片图像堆栈，而无需轴向扫描。我们通过对小鼠大脑中的神经元活动进行钙成像和对心脏样本中的心肌细胞进行电压成像，证明了我们的系统在监测生物样本中的快速动态方面的适用性。