Mechanical properties of cells and tissues closely link to their architectures and physiological functions. To obtain the mechanical information of submillimeter scale small biological objects, we recently focused on the object vibration responses when excited by a femtosecond laser-induced impulsive force. These responses are monitored by the motion of an AFM cantilever placed on top of a sample. In this paper, we examined the surface cellular stiffness of zebrafish embryos based on excited vibration forms in different cytoskeletal states. The vibration responses were more sensitive to their surface cellular stiffness in comparison to the Young’s modulus obtained by a conventional AFM force curve measurement.

中文翻译：

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脉冲激光激活的脉冲响应编码器（PLAIRE）：对斑马鱼胚胎表面细胞硬度的敏感评估

细胞和组织的机械特性与其结构和生理功能密切相关。为了获得亚毫米规模的小型生物物体的机械信息，我们最近集中在由飞秒激光诱导的脉冲力激发时物体的振动响应。这些响应通过放置在样品顶部的AFM悬臂的运动进行监控。在本文中，我们基于不同细胞骨架状态下的激发振动形式检查了斑马鱼胚胎的表面细胞刚度。与通过常规AFM力曲线测量获得的杨氏模量相比，振动响应对其表面细胞刚度更敏感。