Aims: Triple-negative breast cancer (TNBC) represents a specific group that lacks the expression of estrogen receptors, progesterone receptors, and human epidermal growth factor receptor-2 and might also lack the expression other breast markers like GATA3, mammaglobin (MG), GCDFP15 (growth cystic disease fluid protein 15), and NYBR1; when this occurs, proving the breast origin of a metastasis is a challenging task. In the present study, we assessed the added value of SOX10 immunohistochemistry to known GATA3, MG, GCDFP15, and NY-BR-1 statuses in a series of CK5-positive primary TNBCs. Methods: Tissue microarrays were made from the formalin-fixed and paraffin-embedded blocks of 120 TNBCs, and 3-4-mm-thick sections were immunostained for SOX10. The cut-off for a positive reaction was at least 10% of tumor cells staining. Results: In our cohort, SOX10 positivity was seen in 82/119 cases, 61, 74, 76, and 82 all of which were GATA3, MG, GCDFP15, and NY-BR-1 negative, respectively. Of the SOX10 negative cases, 12 stained with at least another breast marker. Nevertheless, 25/119 (21%) cases remained negative with all markers assessed. Discussion: SOX10 proved to be the most commonly positive breast marker in our CK5 expressing TNBCs, but the other markers also had some additive value to SOX10.
Pathobiology

中文翻译：

---

SOX10 免疫组织化学对其他乳腺标志物的附加价值，用于识别源自乳腺的细胞角蛋白 5 阳性三阴性乳腺癌

目的：三阴性乳腺癌 (TNBC) 代表一个特定的群体，它缺乏雌激素受体、孕激素受体和人表皮生长因子受体 2 的表达，也可能缺乏其他乳腺标志物的表达，如 GATA3、乳房珠蛋白 (MG)、 GCDFP15（生长囊性病液蛋白 15）和 NYBR1；当这种情况发生时，证明转移的乳腺起源是一项具有挑战性的任务。在本研究中，我们评估了 SOX10 免疫组织化学对一系列 CK5 阳性原发性 TNBC 中已知 GATA3、MG、GCDFP15 和 NY-BR-1 状态的附加值。方法：组织微阵列由福尔马林固定和石蜡包埋的 120 个 TNBC 块制成，并对 3-4 毫米厚的切片进行 SOX10 免疫染色。阳性反应的临界值是至少 10% 的肿瘤细胞染色。结果：在我们的队列中，在 82/119 例中观察到 SOX10 阳性，其中 61、74、76 和 82 例分别为 GATA3、MG、GCDFP15 和 NY-BR-1 阴性。在 SOX10 阴性病例中，12 例至少被另一种乳房标记物染色。尽管如此，25/119 (21%) 例在评估所有标记后仍为阴性。讨论： SOX10 被证明是我们表达 CK5 的 TNBC 中最常见的阳性乳房标志物，但其他标志物也对 SOX10 具有一些附加价值。
病理生物学