Abstract

Tebuconazole (TBZ) and Chlormequat chloride (CCC) combination has been established as highly effective in reducing plant height of lodging prone wheat varieties. In this work, a novel analytical method employing the quick, easy, cheap, effective, rugged and safe (QuEChERS) cleanup technique and LC-MS/MS (liquid chromatography-tandem mass spectroscopy) was developed for simultaneous estimation of TBZ and CCC in wheat grains and harvest stage plant leaves. A total of 10 mL of acetonitrile and 50 mg of primary secondary amine (PSA) sorbent was consumed in the optimized QuEChERS process for leaves and grain samples. The LC-MS/MS analysis was performed using a C-18 column operating under electrospray ionization in positive mode. The QuEChERS approach achieved extraction recoveries in the acceptable range of 70%–120%, for both the compounds and was validated in terms of accuracy, precision, sensitivity and linearity. Persistence study was conducted using Lihocin (CCC 50% SL), Folicur (TBZ 25.9% EC) and their combination tank mix (Lihocin + Folicur-50% SL + 25.9% EC) applied as foliar spray twice in wheat crop (tester tall variety C-306). The results demonstrated that the developed QuEChERS-LCMS/MS is rapid and confirmatory for simultaneous quantification of both the test analytes in wheat crop.

摘要

已经建立了戊唑醇（TBZ）和氯化氯麦草（CCC）的组合，可有效降低倒伏小麦品种的株高。在这项工作中，开发了一种新颖的分析方法，该方法采用快速，简便，廉价，有效，坚固和安全的（QuEChERS）净化技术和LC-MS / MS（液相色谱-串联质谱）技术来同时估算TBZ和CCC。小麦籽粒和收获期植物叶片。在优化的QuEChERS工艺中，叶片和谷物样品消耗了10 mL乙腈和50 mg伯仲胺（PSA）吸附剂。LC-MS / MS分析使用C-18色谱柱在正离子模式下在电喷雾电离下运行。QuEChERS方法的提取回收率在70％–120％的可接受范围内，两种化合物都适用，并在准确性，精密度，灵敏度和线性方面进行了验证。持久性研究是使用小麦粉（试验性高品种）两次喷施Lihocin（CCC 50％SL），Folicur（TBZ 25.9％EC）和它们的混合罐混物（Lihocin + Folicur-50％SL + 25.9％EC）进行叶面喷雾进行的。 C-306）。结果表明，开发的QuEChERS-LCMS / MS快速，可靠，可同时定量小麦作物​​中的两种测试分析物。