ABSTRACT

The Adipoq-Cre transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective Adipoq-Cre transgenic mouse, strong genetic linkage between a gene of interest, Adam10, and the Adipoq-Cre transgene was discovered. Whole-genome sequencing of the Adipoq-Cre transgenic mouse model identified the genomic insertion site within the Tbx18 gene locus on chromosome 9 and this insertion causes a significant decrease in Tbx18 gene expression in adipose tissue. Insertion of genes Kng2, Kng1, Eif4a2 and Rfc4 also occurred in the Adipoq-Cre transgenic mouse, and these passenger genes may have functional consequences in various tissues.

 抽象的

Adipoq-Cre转基因小鼠广泛用于开发脂肪细胞特异性基因操作，以研究肥胖和 2 型糖尿病。在利用脂肪细胞选择性Adipoq-Cre转基因小鼠开发新小鼠模型的过程中，发现了感兴趣的基因Adam10和Adipoq-Cre转基因之间的强遗传联系。 Adipoq-Cre转基因小鼠模型的全基因组测序确定了第 9 号染色体上Tbx18基因座内的基因组插入位点，该插入导致脂肪组织中Tbx18基因表达显着降低。 Kng2、Kng1、Eif4a2和Rfc4基因的插入也发生在Adipoq-Cre转基因小鼠中，这些过客基因可能在多种组织中产生功能后果。