Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH†,Photochemistry and Photobiology

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Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH†
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-02-09 , DOI: 10.1111/php.13396
Olga Kravchenko ₁ , Todd C Sutherland ₁ , Belinda Heyne ₁

Affiliation

In the scientific literature, the term aqueous environment is loosely employed as it encompasses a broad range of different buffering agents. While there is an increasing number of experimental evidence that point toward specific buffer effects extending far beyond pH, the impact of the chemical nature of the buffering ions is often disregarded, especially in photochemical studies. Herein, we highlighted the importance of buffer specific effects on both the photobleaching and the singlet oxygen quantum yields of a dye in aqueous environments. For this study, we chose erythrosine B (EB) as our model photosensitizer as its photochemistry and photobleaching are well documented in the literature. We followed EB’s photobleaching via absorption spectroscopy in four different aqueous solvents, including pure water, phosphate, Tris and HEPES buffer. These buffer systems were selected because they are commonly used in biochemical and biological applications. Our results show that specific buffer effects cannot be neglected. Indeed, the singlet oxygen quantum yield for EB is significantly different in HEPES compared to the other solvents. Furthermore, we showed that EB’s photoproduct is highly dependent on the nature of the chemical buffer being used.

中文翻译：

水环境中赤藓红 B 的光漂白超过 pH 值†

在科学文献中，水环境这个术语被广泛使用，因为它涵盖了范围广泛的不同缓冲剂。虽然越来越多的实验证据表明特定的缓冲作用远远超出了 pH 值，但缓冲离子的化学性质的影响往往被忽视，尤其是在光化学研究中。在这里，我们强调了缓冲液对水环境中染料的光漂白和单线态氧量子产率的影响的重要性。对于这项研究，我们选择赤藓红 B (EB) 作为我们的模型光敏剂，因为它的光化学和光漂白在文献中有很好的记载。我们通过吸收光谱在四种不同的水性溶剂（包括纯水、磷酸盐、Tris 和 HEPES 缓冲液）中跟踪 EB 的光漂白。选择这些缓冲系统是因为它们通常用于生化和生物学应用。我们的结果表明，特定的缓冲效应不容忽视。实际上，与其他溶剂相比，HEPES 中 EB 的单线态氧量子产率显着不同。此外，我们发现 EB 的光产品高度依赖于所使用的化学缓冲液的性质。

更新日期：2021-02-09

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