In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus‐oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.

中文翻译：

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骨形态发生蛋白 15 补充剂可增强体外成熟牛卵丘卵母细胞复合体的卵丘扩张、核成熟和孕酮产生

与体内相比，体外胚胎生产 (IVP) 效率降低。牛体外卵母细胞成熟 (IVM) 机制的基础知识为提高体外胚胎产量提供了支持。本研究评估了骨形态发生蛋白 15 (BMP15)、成纤维细胞生长因子 16 (FGF16) 及其对卵丘细胞 (CC) 扩增、卵母细胞和 CC DNA 片段化、卵母细胞核成熟、能量代谢和孕酮生成的联合作用。牛 IVM。卵丘-卵母细胞复合物 (COC) 在含有 BMP15 (100 ng/ml)、FGF16 (10 ng/ml) 或 BMP15 与 FGF16 的对照或补充培养基中成熟；并在 IVM 的 0 和 22 小时进行评估。BMP15 单独或其与 FGF16 的关联增强了积云扩张。BMP15 减少了 CC 和卵母细胞中的 DNA 片段化，并提高卵母细胞核成熟率。此外，BMP15 增加了 CC 黄体酮的产生，这是以前未报道过的效果。本研究加强了之前的数据，指出 BMP15 在 IVM 期间的有益影响，同时提供了新的证据，表明潜在的机制涉及增加黄体酮的产生。